Protein Info for ABIE51_RS09105 in Lysobacter sp. OAE881

Annotation: NAD-glutamate dehydrogenase domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 16 to 151 (136 residues), 143 bits, E=2.4e-45
• PF21073: GDH_HM1
  • amino acids 317 to 376 (60 residues), 85.7 bits, see alignment (E = 6.7e-28)
• PF21076: GDH_ACT2
  • amino acids 380 to 470 (91 residues), 104.6 bits, 1.3e-33
• PF21079: GDH_HM2
  • amino acids 477 to 520 (44 residues), 52.5 bits, see alignment (E = 1.6e-17)
• PF21077: GDH_ACT3
  • amino acids 525 to 598 (74 residues), 78.6 bits, 9.6e-26
• PF21078: GDH_HM3
  • amino acids 617 to 682 (66 residues), 103.7 bits, see alignment (E = 1.8e-33)
• PF05088: Bac_GDH_CD
  • amino acids 740 to 1241 (502 residues), 878.8 bits, E=3.9e-268
• PF21074: GDH_C
  • amino acids 1286 to 1622 (337 residues), 407.1 bits, E=2.5e-125

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-alanine degradation II (to D-lactate) (3/3 steps found)
• L-glutamate degradation I (1/1 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• methylaspartate cycle (11/19 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• 4-aminobutanoate degradation V (1/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Protein Sequence (1629 amino acids)

>ABIE51_RS09105 NAD-glutamate dehydrogenase domain-containing protein (Lysobacter sp. OAE881)
MRKQLPKSRHAEGEAFINAFYKRMSSDELPQHDAQGWAELATDFLDFARARKPGAALVRM
FNPTLKGEGWESTHTVIQIANDDMPFLVDSVTMALAEQGIGVHVLGHPVVPFDRDKTGKI
LSVGEGQPESLMHLEIDRQPAEAMPKIQKALEAVLADVRASVRDWPQMKAKMLAVADELG
QRNLPVDEAGKREAQEFLRWAADNHFTFLGYREYEVVQKGGEEVLSAVKSSGMGLLRGKD
VGKPRLLTSLAAHYMPQSGAVDALILTKTNSRATVHRPGYMDYIGVLTFDAKGKPIREER
FLGLYTSSAYNRRPWDIPLVRQRHNYVMQQSGLKPDSHSGKALRHILESLPRDEMFQSSE
EELLRTATGILGLQERVRSKLFLRRDRYGRFFSVLVYIPRDRFNTDVRLRIESMLKRELH
GEHVDSSVTLGGESPLAQVHLIVRPKTGEAIETDSAKIEAELSKLVRNWQDDLRERLVVR
HGEQRGLTLASTYGRALPAGYIEDVSADVAADDVEHLAALSGPDDLRLSLYRAHNGDGGL
RFKFYRQNDDIPLSDALPMMENMGLRVISEHPYRLNANGQTLFVQDFEVETSNPNLDVSS
LDENFEEAFAQIWRGNAENDGFNRLILTGNLSWRQVAMLRGYCKYLLQVGVPFSQSYVEE
TLNRYPLLARLLVELFEAKFDPITGSESKAQIKAGVEAFRAQLSVLAGGDEATLAALGPM
LEARAGKREAQIEATREALKGLFDRVASLDEDRILRSFIGVIDATLRTSYYIQYKDGLRA
DGGPADYISFKFDSSKVPDLPKPRPYREIFVYGPRVEGVHLRFGPVARGGLRWSDRREDF
RTEVLGLVKAQMVKNTVIVPVGSKGGFIAKQLPDPAVNRDAWFNEGVACYKRFINGLLDI
TDNLAGGKVVPPENVVRHDGDDPYLVVAADKGTATFSDIANGIARAHGFWLDDAFASGGS
VGYDHKGMGITARGAWESVKRHFRAMGRDSQKEDFTVVGVGDMSGDVFGNGMLLSKHIRL
VAAFDHRHIFIDPNPDAASTFKERARMFKLPRSSWDDYDRSLISKGGGIYPRSAKSIPLT
AEAKTALGIDPTVSAMSPAELLSAILKAPVDLLWNGGIGTYVKATSETNADVGDRANNAL
RVNGRELRCKVVGEGGNLGMTQLGRIEAAQHGVLLNTDFIDNSAGVDTSDHEVNIKILLN
GEVQKKKLMLPERNKLLAQMTDEVAQLVLTDNYRQNQALSLMERMSVHRLGSKQHFIRTL
ESQGLLDRQIEFLPSDAEIAERKSRGQGLTRPELAVLLSYSKIVLFQQLLDSDVPEDPYL
SKELVRYFPQPLQAKYAKAMEGHRLKREIIATAVTNSMVNRMGATFTLRMTEDTGRTPAE
VAEAYTIAREAVDARDLWAQIDALDGKVPESVQVDALQVIWHLLRSMSRWLLSRPGKMPE
ITAAMARYSDGLNAVRASLPTVLSGMRRDAFEARMNDWKGKGVPAKLAEQLAALPLLEFG
CDIVEISLARKISPVEVAKAYFSLGQALSLPWLYEQVEQLPVDGRWQALARGALRDELAT
QARSLVSQILADGGKKPVEQKVDAWLKRDDSSLRFTLAMFADLHNQKALDYPTLSVAVRR
LAQVAAAGA