Protein Info for ABIE51_RS09085 in Lysobacter sp. OAE881

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 3 to 859 (857 residues), 1227.3 bits, E=0
• PF00809: Pterin_bind
  • amino acids 27 to 265 (239 residues), 205.4 bits, E=2.4e-64
• PF02607: B12-binding_2
  • amino acids 324 to 397 (74 residues), 88.6 bits, E=5.1e-29
• PF02310: B12-binding
  • amino acids 416 to 507 (92 residues), 74.1 bits, E=1.8e-24
• PF02965: Met_synt_B12
  • amino acids 608 to 875 (268 residues), 420.3 bits, E=7.2e-130

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 65% identity to aex:Astex_1859)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (14/18 steps found)
• aspartate superpathway (19/25 steps found)
• folate transformations I (9/13 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• L-methionine biosynthesis I (2/5 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (894 amino acids)

>ABIE51_RS09085 methionine synthase (Lysobacter sp. OAE881)
MSLPRQTRLSGLEPLQITPESNFINVGERTNVTGSAQFKKLILEGRLDEAVVVARQQVEN
GAQVIDVNMDEGLLDSERAMVDFLNLIAAEPDIARVPVMVDSSKFSVIEAGLRCLQGKGI
VNSISMKEGEAEFLRQARLVRRYGAAVVVMAFDEAGQADTMERKVEICSRAYKLLTGTIG
FPPEDIIFDPNVFAIATGIEEHNDYAVAFIEATRELKRRFPFSHISGGVSNVSFSFRGNE
PVRQAIHVVFLYHAIKAGMDMGIVNAGALPLYDDLDAELRERVEDVVLNRRSDATERLLE
IADRYKGKKGEKKVEDLAWREKPVRERLSHSLVHGIDQYIEADTEEARAQATRPLDVIEG
ALMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAYLLPFIEAEKLRTGDVGKSNGKIVM
ATVKGDVHDIGKNIVGVVLACNNFEVIDLGVMVPAQVILDRAKAENADLVGLSGLITPSL
EEMSHVAREMQRQGFEVPLLIGGATTSRAHTALKIDPHYKAPTVWVKDASRAVGVAQSLI
SPDLRGNFVAANDTDYAEIRQRHRQRGDGKRLVSLEKARAQRFDGGWNDYVPPAPNQPGL
HVFDDYPLAELVEYIDWTPFFNTWELAGKYPAILTDEVVGAQASELYRDAQAMLRRIVDE
RWLTAKAVFALWPANATGDDVVVDANGTPTTLHFLRQQVDKPVDRPDFCLADFIAPKESG
RQDWIGGFAVTAGIGIEEHVARFEADHDDYNAILLKALADRFAEALAERLHERVRKEFWG
YAASESLGNDALIDEAYVGIRPAPGYPACPEHSEKATLFRLLDAETNAGVRLTESFAMYP
AAAVSGYYFSHPRSQYFVVGRVSKEQVEDYARRKGVSLAQAERWLASNLDYDPE