Protein Info for ABIE51_RS08915 in Lysobacter sp. OAE881

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 894 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 854 (847 residues), 1217.9 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 505 (474 residues), 567.2 bits, see alignment E=2.4e-174 PF03989: DNA_gyraseA_C" amino acids 537 to 584 (48 residues), 41.3 bits, see alignment 8.4e-15 amino acids 587 to 636 (50 residues), 39.7 bits, see alignment 2.7e-14 amino acids 644 to 688 (45 residues), 34.6 bits, see alignment 1e-12 amino acids 692 to 730 (39 residues), 40.1 bits, see alignment (E = 1.9e-14) amino acids 758 to 802 (45 residues), 47.9 bits, see alignment 7.1e-17 amino acids 809 to 854 (46 residues), 38.5 bits, see alignment 6.1e-14

Best Hits

Swiss-Prot: 67% identical to GYRA_PECCA: DNA gyrase subunit A (gyrA) from Pectobacterium carotovorum

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 63% identity to gan:UMN179_02454)

MetaCyc: 67% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (894 amino acids)

>ABIE51_RS08915 DNA gyrase subunit A (Lysobacter sp. OAE881)
MAELAKEIIPVNLEDEMRRSYLDYAMSVIVGRALPDARDGLKPVHRRVLYAMNELGAHSN
KPYYKSARIVGDVIGKYHPHGDTAVYDTLVRMAQPFSLRYMLVDGQGNFGSIDGDNAAAM
RYTEARMSRIAHELMADIDKETVDFQPNYDEKELEPTVMPTRVPNLLVNGSAGIAVGMAT
NIPPHNLSEVIDATIALIDEPTIDIDGLLQYIPGPDFPTAGIINGTAGITAAYRTGRGRV
RMRARAEVEVADNGREAIVVTEIPYQVNKARLIEKIAELVKEKKLEGISELRDESDKDGM
RIYIEVKRGESAEVVLNNLYQQTQMESVFGINMVAIVDGRPKLLNLKELLEVFVKHRREV
VTRRTIFELRKARQRAHILEGLTVALANIDEMIELIKTSANPNEARERMLARTWEPGLVG
ALLAAAGSDASRPEDLPNGVGLIDGRYQLTETQAQQILEMRLHRLTGLEQDKLTDEYKQL
LETIRGLIEILEDPSVLLEVIRTELRNVKEEFGDARRSEIRASEEDLDILDLIAPEDVVV
TLSHSGYAKRQPVSAYRAQKRGGKGRNAASTKDEDFIDHLWLVNTHDTLLTFTSAGRVFW
LPVHQLPDAGPNARGRPIINWIPLENGEQVQAVLPVREYEEGRYVFFATRNGTVKKTPLT
EFAFRLQRGKIAINLDEGDALVDVAMTNGDCDIMLFASNGKAVRFSEDAVRSMGRTATGV
RGIRLAEGEEGEEGDDNGGRNTSGAYVVSLIVVEGDGDILTASERGYGKRTALEEYPRKG
RGGQGVIALKTTERNGKLIGAVQLSDHHDVLLISDGGTLVRTRAAEISQVGRNTQGVTLM
RLSAEESLQTIERVDASLDQDEENGGEASIDGTSAVVADASAEASGETDSPAAT