Protein Info for ABIE51_RS08215 in Lysobacter sp. OAE881

Annotation: 50S ribosomal protein L3 N(5)-glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 16 to 295 (280 residues), 375.2 bits, see alignment E=2.1e-116 TIGR00536: methyltransferase, HemK family" amino acids 19 to 303 (285 residues), 226.2 bits, see alignment E=4.5e-71 PF06325: PrmA" amino acids 134 to 208 (75 residues), 29.4 bits, see alignment E=1.7e-10 PF13847: Methyltransf_31" amino acids 136 to 270 (135 residues), 45.5 bits, see alignment E=2e-15 PF05175: MTS" amino acids 136 to 217 (82 residues), 60.4 bits, see alignment E=5.2e-20 PF13649: Methyltransf_25" amino acids 138 to 219 (82 residues), 35 bits, see alignment E=5.8e-12 PF08242: Methyltransf_12" amino acids 139 to 208 (70 residues), 30.1 bits, see alignment E=2e-10

Best Hits

Swiss-Prot: 80% identical to PRMB_XANCP: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 79% identity to psu:Psesu_1950)

MetaCyc: 46% identical to ribosomal protein L3 N5-glutamine methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-1241 [EC: 2.1.1.298]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.298 or 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (313 amino acids)

>ABIE51_RS08215 50S ribosomal protein L3 N(5)-glutamine methyltransferase (Lysobacter sp. OAE881)
MNGSATPNPVTFEQVSIIDLIRYGGSRFNEAGLTFGHSYDNALDEATQLVLHALHLPHDL
SPIYGASRVTAAEQAKVLDLFQRRIDERVPAAYLTGEAWFAGLSFKSDRRALVPRSPIAE
LIPHGYEPWLGGREVERVLDLCTGSGCIAIATAHYHPHWQVDGVDINDDALSLAKENKER
LHADNVTLRKSDLFNQLQGQVYDLIVTNPPYVTNDETDALPKEYSYEPELGLRAGDDGLD
LALKILRDAPDHLTENGLLICEVGEAERALVELLPELPMAWVEFKVGQMGIFVVERADLV
EHHAKIKALADAR