Protein Info for ABIE51_RS08085 in Lysobacter sp. OAE881

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 690 PF00133: tRNA-synt_1" amino acids 3 to 63 (61 residues), 37.6 bits, see alignment 2.4e-13 TIGR00398: methionine--tRNA ligase" amino acids 6 to 535 (530 residues), 526.4 bits, see alignment E=8.9e-162 PF09334: tRNA-synt_1g" amino acids 6 to 395 (390 residues), 485.3 bits, see alignment E=2.4e-149 PF19303: Anticodon_3" amino acids 424 to 521 (98 residues), 28.7 bits, see alignment E=2.3e-10 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 561 to 689 (129 residues), 129.4 bits, see alignment E=8.1e-42 PF01588: tRNA_bind" amino acids 593 to 687 (95 residues), 81.3 bits, see alignment E=9.3e-27

Best Hits

Swiss-Prot: 80% identical to SYM_STRM5: Methionine--tRNA ligase (metG) from Stenotrophomonas maltophilia (strain R551-3)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 80% identity to smt:Smal_2926)

MetaCyc: 55% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (690 amino acids)

>ABIE51_RS08085 methionine--tRNA ligase (Lysobacter sp. OAE881)
MSREFVVSCALPYANGQLHLGHLVGYTQADIWCRAQRMAGHKAWYVCADDTHGTPIMLAA
EKAGQSPEAFIAGIQQSHERDFADFGVAFDHYDSTNSERNRVLTEAIYAKLDNNGHISRR
SVAQLFDPVKGMFLPDRYVKGICPNCGTPDQYGDNCENCGATYSPTELKEPRSVVSGATP
ELRESEHFFFEVGQFEGFLRQWLAGDVALPGVKAKLQEWLDAEGGLRAWDISRDAPYFGF
QIPGHPGKYLYVWLDAPIGYLSSFQALCEREGLDFWSYLRRDSPAELHHFIGKDIVNFHG
LFWPAVLHGAGFRAPTRLHVNGYLTVDGAKMSKSRGTFVMARTYLDAGLHPEALRYYYAT
KTTGGVDDLDLNLADFVARVNADLVGKFVNLASRCAGFIEKRFDGRLADALPDAAQYVRF
VEQLEPIAQAYARNEAATALRLTMALADEANKYIDEHKPWVLAKQDGADAQLQAVCTQGL
NLFRVLNTALKPVLPRVTAQAEDFLAAKVANWSELAAPLLAHRINAYAPLFTRIDPKQID
AMIEASKDSLQSAPKNESETKAEAKPAKPAAAPAPSAGETATIGIDDFAKLDLRVGKVVA
CEFVDGSDKLLRFELDAGELGTRQIFSGIRGSYGEPEKLVGRSVVFIANLAPRKMRFGIS
EGMILSAGFDGGSLHLLDADAGVQPGAPVR