Protein Info for ABIE51_RS07650 in Lysobacter sp. OAE881

Annotation: bifunctional riboflavin kinase/FAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 14 to 168 (155 residues), 161.8 bits, E=1.2e-51
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 20 to 307 (288 residues), 268.4 bits, E=4.1e-84
• PF01687: Flavokinase
  • amino acids 185 to 307 (123 residues), 136.1 bits, E=7.7e-44

Best Hits

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 72% identity to psu:Psesu_0975)

MetaCyc: 39% identical to bifunctional riboflavin kinase/FAD synthase (Streptomyces davaonensis)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]; 2.7.1.26 [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin salvage (2/2 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (1/3 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (19/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Protein Sequence (316 amino acids)

>ABIE51_RS07650 bifunctional riboflavin kinase/FAD synthetase (Lysobacter sp. OAE881)
MSRLFRDVEGGPRCPHGSVVCIGAFDGLHLGHQALVRHALARARALDVPCVVLSFEPLPR
EFFAPNDPPPRLMLPRTKAGRLFEMGVDQLGLLRFDRRLCSLSAEEFAEQVLVHRLNARE
VWVGPEFRFGKARGGDIGVLKAVGQAHGFVAGEIEPVQVDGERVSATRIRQALREGDFAT
AERFLGRPYAIAGHVVQGKQLGRTLGFPTANLRFGGRTPALSGIYATWVHGVGDAPRASV
SSLGTRPTVDGVEPLLEAHLFDFDGDLYGRRIEVEFVAKLRDELKFSDLPALTAQMHRDA
AEAREILSKEQHHAFA