Protein Info for ABIE51_RS07430 in Lysobacter sp. OAE881

Annotation: NAD-dependent epimerase/dehydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08659: KR
  • amino acids 15 to 97 (83 residues), 23.5 bits, E=1.7e-08
• PF04321: RmlD_sub_bind
  • amino acids 16 to 260 (245 residues), 35.5 bits, E=2.2e-12
• PF02719: Polysacc_synt_2
  • amino acids 16 to 140 (125 residues), 42.2 bits, E=2e-14
• PF01370: Epimerase
  • amino acids 16 to 277 (262 residues), 165.8 bits, E=4.1e-52
• PF16363: GDP_Man_Dehyd
  • amino acids 17 to 341 (325 residues), 147.9 bits, E=1.8e-46
• PF01073: 3Beta_HSD
  • amino acids 17 to 265 (249 residues), 55.1 bits, E=2.2e-18
• PF07993: NAD_binding_4
  • amino acids 18 to 227 (210 residues), 54.6 bits, E=3.2e-18

Best Hits

KEGG orthology group: K12454, CDP-paratose 2-epimerase [EC: 5.1.3.10] (inferred from 63% identity to afw:Anae109_3217)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• colanic acid building blocks biosynthesis (8/11 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• CDP-tyvelose biosynthesis (1/2 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis (1/8 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.10 or 5.1.3.2

Protein Sequence (366 amino acids)

>ABIE51_RS07430 NAD-dependent epimerase/dehydratase family protein (Lysobacter sp. OAE881)
MNARTHLHEPTNEGPVLITGGAGFIGTNLATRLLESGRRVVVLDNLSRPGVEANAHWLWH
RFGDRVAIEIGDVRDAGRVRGLVANASQVFHFAAQVAVTSSLVDPRDDFETNLGGTLNVL
EAMRATETPPSLVFTSTNKVYGALDDIALDRRGDRYAPHDETLCSAGVSEARPLDFHSPY
GCSKGGADQYVLDYARSFGLPAALFRMSCIYGPHQCGNEDQGWVAHFLRCALRREPITVY
GDGRQVRDILFVTDLADAFVRCQARMHELAGRAFNMGGGPSNALSLREVLGTIASLTGRA
PQIEYGPWRVGDQRWYVSDTQAFRRATGWAPAVDAQEGIARLHEWLVETTMPSQARVPAT
SRTHAA