Protein Info for ABIE51_RS06100 in Lysobacter sp. OAE881

Annotation: bifunctional 2-methylcitrate dehydratase/aconitate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 TIGR02330: 2-methylcitrate dehydratase" amino acids 15 to 485 (471 residues), 708.8 bits, see alignment E=1.5e-217 PF03972: MmgE_PrpD_N" amino acids 20 to 271 (252 residues), 269.1 bits, see alignment E=3e-84 PF19305: MmgE_PrpD_C" amino acids 289 to 464 (176 residues), 178.3 bits, see alignment E=1.2e-56

Best Hits

Swiss-Prot: 57% identical to PRPD_CUPNE: 2-methylcitrate dehydratase (prpD) from Cupriavidus necator

KEGG orthology group: K01720, 2-methylcitrate dehydratase [EC: 4.2.1.79] (inferred from 63% identity to hha:Hhal_1070)

Predicted SEED Role

"2-methylcitrate dehydratase (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.79

Use Curated BLAST to search for 4.2.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (487 amino acids)

>ABIE51_RS06100 bifunctional 2-methylcitrate dehydratase/aconitate hydratase (Lysobacter sp. OAE881)
MSLAQVRSAVRPPPDEAMTQVADYVVRSDVGSREAYETARFMLLDSLACAALAMDHEQCV
NHLGPLVPGAELRDGARVPYTAFELDPAQAAYNIGTQIRWLDFNDTWLAAEWGHPSDNLG
AILAVADYLGQQAIAQGRAPMTVRDVLGWAIKAHEIQGCYALKNAFNRVGLDHVILVRLA
STAVATAMLGGSRDQIVTAISQSWIDNGALRTYRHAPNTGPRKSWAAGDACRRAVIHALN
AMKGVVGYPSALTAPTWGFYDVAFGGKPFEFERDFGSYVMENVLFKISYPAEFHAQTAVE
CAMQLHPLVAPRIGRDGGESVERIVIETQQAALRIIDKTGPLANYADRDHCIQYMVAVPL
LFGRLTAQDYTDAVAADPRIDLLRGRTVVMENPRFTQEYFDPDKRYIGNSVQVHFKDGAS
TPKVSIDYPIGHRRRRDEGMAVLMKKFESAIASKLPEIQARTLMTLASSPGGFDAMPITQ
FMSLFKK