Protein Info for ABIE51_RS06045 in Lysobacter sp. OAE881

Annotation: NADPH-dependent 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 PF00724: Oxidored_FMN" amino acids 24 to 348 (325 residues), 255.1 bits, see alignment E=5.9e-79 PF00070: Pyr_redox" amino acids 394 to 431 (38 residues), 22.4 bits, see alignment 7.2e-08 PF03486: HI0933_like" amino acids 394 to 432 (39 residues), 27.9 bits, see alignment 5e-10 PF07992: Pyr_redox_2" amino acids 394 to 653 (260 residues), 83.9 bits, see alignment E=7.1e-27 PF01134: GIDA" amino acids 395 to 448 (54 residues), 21.8 bits, see alignment 4.5e-08 PF12831: FAD_oxidored" amino acids 395 to 431 (37 residues), 34.6 bits, see alignment 7.1e-12 PF00890: FAD_binding_2" amino acids 395 to 433 (39 residues), 25.3 bits, see alignment 4.4e-09 PF13450: NAD_binding_8" amino acids 397 to 435 (39 residues), 35.4 bits, see alignment 5.3e-12

Best Hits

Swiss-Prot: 60% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 77% identity to sml:Smlt0952)

MetaCyc: 60% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (689 amino acids)

>ABIE51_RS06045 NADPH-dependent 2,4-dienoyl-CoA reductase (Lysobacter sp. OAE881)
MTIANPAGPLHFDEAEPHPLYPHLFAPLDLGFCTLPNRVLMGSMHTGLEDKAADFPKLAA
YFAERAAGGVGLIVTGGFSPNLAGWLKPFGGTMRFPWEVRRHRQVTGAVHAHGGRICLQI
LHAGRYGYSPLQVAPSRLKAPINPFTPRALSAGGVERQIRAFVRSATMAREAGYDGVEVM
GSEGYLLNQFTAPRTNKRHDAWGGDAARRMRFAVEIVRRIREACGPDFIIVYRLSMLDLV
DDGLAWDEVVQQAKAIEAAGATLINTGIGWHEARVPTIATSVPRAAFTGITARLKPHVGV
PLVTTNRINMPEVAEGVLARGEADMVSMARPLLADPQWVNKARERRALDINTCIACNQAC
LDHVFENKTASCLVNPRACAETELNYLPTKNPKRIAVVGAGPAGLAAATVAAERGHQVTL
FEATGEIGGQFNLAKRIPGKEEFHETLRYFGRKLEQTGVDVRLGTYADAAMLAGFDAVVL
ASGIRPREVDFPGADHAKVVSYLDVLTGRAVPGRRVAVVGAGGIGFDVAEFLVEHGPSAA
LDAARWRAEWGVDLGYDGNRGGLAAPKPEAPAREVWLLQRSSGRPGARLGKTTGWIHRAT
LKAKKVRMLGGVEYLGVDDEGFHVRVDGSEQVLPVDHVVICAGQEPFKPLADELAANGAT
VHVIGGADVAAELDAKRAIAQGSRVASML