Protein Info for ABIE51_RS05015 in Lysobacter sp. OAE881

Annotation: aspartate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 599 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 592 (592 residues), 756.4 bits, see alignment E=9.5e-232 PF01336: tRNA_anti-codon" amino acids 18 to 110 (93 residues), 49.2 bits, see alignment E=6.2e-17 PF00152: tRNA-synt_2" amino acids 126 to 567 (442 residues), 393.7 bits, see alignment E=9e-122 PF02938: GAD" amino acids 320 to 413 (94 residues), 84.9 bits, see alignment E=5.8e-28

Best Hits

Swiss-Prot: 77% identical to SYD_XANCB: Aspartate--tRNA ligase (aspS) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 77% identity to xca:xccb100_1166)

MetaCyc: 57% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (599 amino acids)

>ABIE51_RS05015 aspartate--tRNA ligase (Lysobacter sp. OAE881)
MRTHFCGLVDEALIGQTVTLCGWADVARNLGGLCFIDLRDHEGIVQIVAEPTTDAALGAG
EANAAVIATAANVGYEDCLRVTGVVRKRESVNAKIRTGQVEVVATKIEVLNKAEPLPFHA
HENPGEDIRLKYRYLDMRRPEMQKMMRTRIKLVQALRRYLDERGFQDIETPILTKATPEG
ARDFLVPARMHPGEFYALPQSPQLFKQILMMAGFDRYYQIARCFRDEALRADRQLEFTQL
DMEFAWVSERDVQDTVEEMIRVVFRDVMGVELAAQFPRMTYAEAMRRYGSDKPDLRIALE
FTDIAQLVKSCEFKVFTDWANHEDGRVVALRVPGASTLSRKQIDDYAAYAGKFGAKGLAW
MKVEDASKGREGINSPIAKFLDDATLAAIVQATGATTGDAIFFGAATYKSASDFMGALRL
KVAKDLGLVAEGWAPLWVTDFPMFEWDDEENRYVALHHPFTAPAVDSIDDLRANAKTAVS
RGYDMVLNGNEIGGGSIRIHNSQMQSAVFDLLGIGAEEAEGKFGFLLDALKFGAPPHGGI
AFGIDRIAALMAGTESIRDVIAFPKTTTAQCLMTGAPSPVPDKQLAEVHIQIREKQEGK