Protein Info for ABIE51_RS04465 in Lysobacter sp. OAE881

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 927 transmembrane" amino acids 483 to 504 (22 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 7 to 908 (902 residues), 1115.1 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 18 to 609 (592 residues), 463.3 bits, see alignment E=1.7e-142 PF09334: tRNA-synt_1g" amino acids 44 to 90 (47 residues), 26.6 bits, see alignment 6.3e-10 amino acids 337 to 427 (91 residues), 26.3 bits, see alignment E=8.1e-10 PF13603: tRNA-synt_1_2" amino acids 243 to 304 (62 residues), 33.6 bits, see alignment 7.3e-12 PF08264: Anticodon_1" amino acids 650 to 801 (152 residues), 135.4 bits, see alignment E=3.6e-43 PF10458: Val_tRNA-synt_C" amino acids 862 to 926 (65 residues), 66.6 bits, see alignment 4.9e-22

Best Hits

Swiss-Prot: 58% identical to SYV_NITEU: Valine--tRNA ligase (valS) from Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 58% identity to neu:NE0444)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (927 amino acids)

>ABIE51_RS04465 valine--tRNA ligase (Lysobacter sp. OAE881)
MTHTLASSYDPKQFETRLYQEWESSGVFQPRGEGEPYSILLPPPNVTGTLHMGHAFQHTL
MDALIRYHRMRGYDTLWQMGTDHAGIATEMVVSRNLAAEGAGETRDSLGRDGFISKVWEW
KQQSGDTIEKQMRRIGASGDWSRSVFTMDPMAANAVIEAFVRLYEDGLIYRGQRLVNWDP
VLKTAISDLEVVNEEEDGFLWSISYPLTDGSGELTVATTRPETMLGDVAVMVHPQDERYT
HLIGRTVTLPLTGREIPVIADDYVDREFGTGVVKVTPAHDFNDYAVGQRHNLELINIFTP
TAAVNENAPEKYRGLDRYEARKVVLADLEAEGRLVETKPHKLQVPRGDRTGQVIEPYLTD
QWFVKMDGLAARGLELVEKGDVKFVPANWINTYRHWMENIQDWCISRQLWWGHRIPAWYD
DDGKIYVGRTEEEVRAKQGLGSNVTLSQDSDVLETWFSSQLWPFSTMGWPDEQAMAERGF
DRYVPSSVLVTGFDIIFFWVARMIMATDQFTGRVPFRDVYVTGLIRDSHGQKMSKSKGNV
LDPLDLIDGISLDDLVAKRTGGLMQPKMAEKIEKATRKEFPDGIPAFGADALRFTIAALA
THGRDIKFDLGRAEGYKNFCNKLWNATRFVLMNTEGANFIGVPDPKTDAERWILSRLAKV
AAEAEQHFAGYRFDLLSQALYEFAWNEFCDWFVELAKPALQGEDAIAANSTRHTLLFVLE
RLLSLLHPLIPFVTEELWQQVAPKLGVGEKTIMLRPYPQASDFAGQDYAAAEGDVEWLKA
MVTSLRKIRSELNVAPSKTVPLLVADCSASDRERVARFDSQLRFLTRLDGITVLDDANAA
PASATALVGELKLFVPLEGLVDLGAERARLDKELKRVEGEIAKCHNKLASETFVANAPPA
VVEQERKRLADWNLQREALAEQRAKLA