Protein Info for ABIE51_RS03875 in Lysobacter sp. OAE881

Annotation: oxygen-independent coproporphyrinogen III oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 TIGR00538: oxygen-independent coproporphyrinogen III oxidase" amino acids 7 to 463 (457 residues), 563 bits, see alignment E=2.4e-173 PF04055: Radical_SAM" amino acids 59 to 233 (175 residues), 84.1 bits, see alignment E=1.3e-27 PF06969: HemN_C" amino acids 369 to 438 (70 residues), 38.8 bits, see alignment E=7.8e-14

Best Hits

Swiss-Prot: 52% identical to HEMN_PSEAE: Oxygen-independent coproporphyrinogen III oxidase (hemN) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 67% identity to psu:Psesu_0365)

MetaCyc: 50% identical to coproporphyrinogen III dehydrogenase (Escherichia coli K-12 substr. MG1655)
HEMN-RXN [EC: 1.3.98.3]

Predicted SEED Role

"Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.99.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.98.3 or 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (464 amino acids)

>ABIE51_RS03875 oxygen-independent coproporphyrinogen III oxidase (Lysobacter sp. OAE881)
MSTAPTFPAELLRRYDRPGPRYTSYPTAPQFSPDFDIAQLRDAAQASNGDPIPSRLSLYV
HVPFCTNPCFYCGCNRVITRDRARSEPYRTRLAREIGMMAGLFDPDREVVQLHFGGGTPN
FFTPAQLRATVQDLRDHFRFSVDSQRDISIELDPRELTPDDIAELADVGFNRASLGVQDF
DPDVQRAVNREQDVGATLAIIDACRAHGMRSVNVDLIYGLPLQTLDGFSRTLDIVIAARP
HRLAVYSYAHLPELFKPQRQIRAEDLPSNELKLDLLRLAIERLTAAGYVYIGMDHFALPD
DELALAQARGGLHRNFMGYTTHADCDLIGFGVSAISRIGDSYSQNPRELPAWEEAIDAPR
LPVWRGLRLSPDDQLRADVIQRLMCQGEVSMGAIERAHGIDFTHYFADALARLQPLQDDG
LVWIEPDRIVATREGRLLLRVIAMCFDAYLRQPHATSAKYSRVV