Protein Info for ABIE51_RS02220 in Lysobacter sp. OAE881

Annotation: DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 832 PF01751: Toprim" amino acids 5 to 117 (113 residues), 73.1 bits, see alignment E=2.6e-24 TIGR01051: DNA topoisomerase I" amino acids 5 to 618 (614 residues), 755.1 bits, see alignment E=4.4e-231 PF01131: Topoisom_bac" amino acids 133 to 562 (430 residues), 440.9 bits, see alignment E=7.8e-136 PF13368: Toprim_C_rpt" amino acids 594 to 653 (60 residues), 52.6 bits, see alignment 5.5e-18 amino acids 657 to 702 (46 residues), 48 bits, see alignment 1.5e-16 amino acids 715 to 759 (45 residues), 51.3 bits, see alignment 1.4e-17

Best Hits

Swiss-Prot: 77% identical to TOP1_XYLFA: DNA topoisomerase 1 (topA) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 84% identity to smt:Smal_3591)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (832 amino acids)

>ABIE51_RS02220 DNA topoisomerase I (Lysobacter sp. OAE881)
MAKNLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPERGFAMRYDLIDKN
EKHVDAIAKAAKGADALYLATDPDREGEAISWHIAEILKERGLLEGKTLQRVVFTEITPR
AIKEAMTQPRAIASDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIV
EREEEIEAFVAREYWTIEAECAHPTQAFTAKLIKLDGKKFEQFTVTDGDTAEDARKRITA
AANGALHVTDVASKERKRRPAAPFTTSTLQQEASRKLGFTTRKTMQVAQKLYEGVAIGEE
GTVGLITYMRTDSVTLSQDAIGEIRDVIARDYGTRALPDKPNTYQNKSKNAQEAHEAVRP
TSALRTPSSVARYLSDDERKLYELVWKRAVASQMVPATLNTVSVDLAAGSEHAFRASGTT
VVDPGFLAAYEEGKDTRTADDEDEGRKLPAMKTGDRVPLDRIHADQHFTQPPPRYTEAAL
VKALEEYGIGRPSTYASIIQTLLFRKYVEMEGRSFRPSDVGRAVSKFLSSHFTRYVDYDF
TAKLEDELDAVSRGEEEWVPLMEKFWGPFKELVEDKTESVDRSEATGARELGTDPKSGKP
VSVRLGRFGPYAQIGDKDTDEKLEFASLRPGQSMHTITLEDALELFKLPRKLGENAGHEV
SVGIGRFGPFAKRGTTYASLTKEDDPYTIDLARAVFLIEQKEEIARNRIIRQWDDSDVQV
LNGRFGPYISDGKLNGKIPKDREPASLSLEEATQFLADTGKPVRRGFGKKAAKKEPAVKV
KKEAAAKKAPAAKKAPAKKAAAKKTAKKAAKKTAAKAPAKTAAKKVVKKAKA