Protein Info for ABIE51_RS01985 in Lysobacter sp. OAE881

Annotation: DNA helicase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 730 TIGR01075: DNA helicase II" amino acids 6 to 727 (722 residues), 1170.2 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 10 to 272 (263 residues), 289 bits, see alignment E=1.1e-89 PF13245: AAA_19" amino acids 14 to 256 (243 residues), 81.1 bits, see alignment E=2.1e-26 PF13361: UvrD_C" amino acids 278 to 437 (160 residues), 146.6 bits, see alignment E=3.2e-46 amino acids 499 to 619 (121 residues), 117.7 bits, see alignment E=2.1e-37 PF13538: UvrD_C_2" amino acids 559 to 615 (57 residues), 32.7 bits, see alignment 1.4e-11 PF21196: PcrA_UvrD_tudor" amino acids 683 to 725 (43 residues), 29 bits, see alignment 2e-10

Best Hits

Swiss-Prot: 62% identical to UVRD_ECOLI: DNA helicase II (uvrD) from Escherichia coli (strain K12)

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 79% identity to psu:Psesu_2815)

MetaCyc: 62% identical to DNA helicase II (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (730 amino acids)

>ABIE51_RS01985 DNA helicase II (Lysobacter sp. OAE881)
MDVSHLLDALNPAQREAVSAPPGHYLVLAGAGSGKTRVLTHRIAWMNEVHGVPTHGILAV
TFTNKAAGEMRHRVDGQLRHGARGMWIGTFHGLAHRLLRLHWQEAKLPEGFQVLDSDDQL
RLVKRVVQALEFDETRFPPRQIVWWINAQKDEGRRPHNLQPTSGDPWADVMHKCYEAYQE
RCERAGLVDFAELLLRAHELLRDNPALLAHYRHRFREILVDEFQDTNAIQYAFVRVLAGD
TGHVFVVGDDDQSIYGWRGAKVENMQRFLKDFSGTHTIRLEQNYRSSANILEAANAVIAH
NPDRLGKNLWTDSGTGEPIDLYAAYNEMDEARFVVERLRQWVRDGGSYGEVAILYRSNAQ
SRAFEESLLSEQVPYRVYGGLRFFERAEIKDTLAYLRLIASRLDDAAFERAVNTPTRGIG
ERTLDEVRRRARADGVSLWEASRRIVGENGLAARARNAVAGFLAMIDDLDNELASMTLPE
KIDHVLIRSGLREHYFNESRGQLDSRVDNLDELVSVASRFTRTDDEDAAAMPELVAFLSY
AALEAGEGQAQAGEEGVQLMTLHSAKGLEFPLVFLAGMEEGVFPSGRSTEESGRLEEERR
LAYVGITRARQKLVLSYAESRRIHGADMYGVPSRFLREIPAPLLNEVRPKVQVSRPYSGG
VQRGVLGASRGHAPLEQPAVRLGSQVKHPSFGMGTVTDYEGSGAHARVQVNFDDAGSKWL
VLAYANLQPA