Protein Info for ABIE51_RS01660 in Lysobacter sp. OAE881

Annotation: acetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 22 to 644 (623 residues), 1013.3 bits, E=0
• PF16177: ACAS_N
  • amino acids 25 to 82 (58 residues), 68 bits, 7.3e-23
• PF00501: AMP-binding
  • amino acids 84 to 467 (384 residues), 300.1 bits, E=3.2e-93
• PF13193: AMP-binding_C
  • amino acids 533 to 611 (79 residues), 93.2 bits, E=2.3e-30

Best Hits

Swiss-Prot: 84% identical to ACSA_XANC8: Acetyl-coenzyme A synthetase (acsA) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 85% identity to psu:Psesu_0239)

MetaCyc: 62% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• acrylate degradation II (3/3 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• superpathway of acetate utilization and formation (2/3 steps found)
• 2-methylcitrate cycle II (4/6 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (6/10 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• β-alanine biosynthesis II (2/6 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Protein Sequence (650 amino acids)

>ABIE51_RS01660 acetate--CoA ligase (Lysobacter sp. OAE881)
MNHPAAVYPIDPDFAANARVTREDYERQYAESVNDPAGFWGKVADRLDWFTKPTKIKDVS
YDLADFRIRWYEDGELNASVNCLDRHLDKRGDKTALIFEHDDPSQPAERITYRELHARVC
KLANALRSLGVRKGDRVTIYLPMIPEAVVAMLACARVGAIHSVVFGGFAPQSIADRVADC
QSKLIITADEGLRGGKKVPLKANVDAALKLGGTNTVETVLVVRHTGSAVDMQMPRDRWYD
AVIDEQPTQCAPERMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASYTHEVVFDLRED
DVYWCTADVGWVTGHSYIVYGPLANGATALVFEGVPNYPTVSRFWEVIDKHQVTLFYTAP
TAIRALMRDGDEPVKKTSRASLRLLGTVGEPINPEAWRWYYEVVGDSRSPIVDTWWQTET
GGILITPLAGAIDLKPGSATKPFFGIQPALVDANGGRLEGEAEGNLVLLDSWPGQMRTVY
GDHARFIDTYFRTYPGTYFTGDGCRRDADGYYWITGRVDDVINVSGHRIGTAEVESALVS
HPKVAEAAVVGFPHDIKGQGIYAYVTLIAGEAPTDELHKELVAHVRKEIGPIATPDHLQW
APGLPKTRSGKIMRRILRKIAENAPDQLGDTSTLADPAVVESLVRERRVP