Protein Info for ABIE51_RS01245 in Lysobacter sp. OAE881

Annotation: glycine--tRNA ligase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 TIGR00388: glycine--tRNA ligase, alpha subunit" amino acids 10 to 302 (293 residues), 518.6 bits, see alignment E=2.2e-160 PF02091: tRNA-synt_2e" amino acids 11 to 293 (283 residues), 480.8 bits, see alignment E=6e-149

Best Hits

Swiss-Prot: 89% identical to SYGA_XANCB: Glycine--tRNA ligase alpha subunit (glyQ) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K01878, glycyl-tRNA synthetase alpha chain [EC: 6.1.1.14] (inferred from 91% identity to psu:Psesu_2962)

MetaCyc: 73% identical to glycine--tRNA ligase subunit alpha (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (307 amino acids)

>ABIE51_RS01245 glycine--tRNA ligase subunit alpha (Lysobacter sp. OAE881)
MAAPTFQGPTFQELIQRLNAYWADQGCTLIQPLDLEVGAGTFHPATFLRALGPEPWNAAY
VQPCRRPTDGRYGENPNRLQRYYQYQVAMKPSPDNIVELYFDSLKALGVDPLVHDLRLVE
DNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQAGGLECRPVLGEITYGLERLCMYLQNVD
NVYDLIWTYGPDGTPVTYGDVYHQNEVEQSAYNFEHADVAELFHRFDACEAEALKLIELG
LPLPAYDQVCKASHSFNLLDARRAISVTERQRYILRVRRVAQGVAEAYYAQREKLGFPVL
KKAAKAA