Protein Info for ABIE51_RS00735 in Lysobacter sp. OAE881

Annotation: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 PF10396: TrmE_N" amino acids 6 to 119 (114 residues), 133.7 bits, see alignment E=7.6e-43 TIGR00450: tRNA modification GTPase TrmE" amino acids 11 to 445 (435 residues), 333.9 bits, see alignment E=1.8e-103 PF12631: MnmE_helical" amino acids 122 to 442 (321 residues), 181.9 bits, see alignment E=3.7e-57 PF02421: FeoB_N" amino acids 218 to 308 (91 residues), 29.8 bits, see alignment E=8e-11 PF01926: MMR_HSR1" amino acids 218 to 309 (92 residues), 80.6 bits, see alignment E=1.9e-26 TIGR00231: small GTP-binding protein domain" amino acids 219 to 342 (124 residues), 77.5 bits, see alignment E=1e-25

Best Hits

Swiss-Prot: 70% identical to MNME_XANOR: tRNA modification GTPase MnmE (mnmE) from Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 70% identity to xoo:XOO4634)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (445 amino acids)

>ABIE51_RS00735 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE (Lysobacter sp. OAE881)
MTDRDTIAAIATAPGAGGVGIVRLSGPKSRAIAQSLAAHELVPRTAHYVRFRDEHYETID
DGIALYFAAPASYTGEDVVELQAHGSPAVLHELVARCVALGARRARPGEFSERAFLEGRL
DLAQAEAVADLIAAADVRAARAARRALDGEFSRRVDAIADELLAIRVHVEAAIDFADEPI
DTLGGEQLRVRLRDNALALDDLLKAAERGRRLRDGLHAVIVGPPNAGKSSLLNVLAGSER
AIVTDIAGTTRDLLHETIRIDGVELTLVDTAGLREGGDVIEREGMRRARGELQRADLALV
VLDARDPEGGRAAVIDAIAEVPLRVWVHNKADLLDADTPQPHDALNVSARTGTGLESLHA
RLRELAQGNADAAGEGAFTARARHVDALAIAREHLAQARIELDRESLDLAAESLRLAHDA
LGEITGRVHADDLLGHIFSSFCIGK