Protein Info for ABIE51_RS00615 in Lysobacter sp. OAE881

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 2 to 148 (147 residues), 79.8 bits, E=1.3e-26
• TIGR00093: pseudouridine synthase
  • amino acids 6 to 177 (172 residues), 186.5 bits, E=1.4e-59

Best Hits

Swiss-Prot: 83% identical to RLUE_XANAC: Ribosomal large subunit pseudouridine synthase E (rluE) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K06181, ribosomal large subunit pseudouridine synthase E [EC: 5.4.99.12] (inferred from 80% identity to psu:Psesu_3070)

MetaCyc: 60% identical to 23S rRNA pseudouridine2457 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11834 [EC: 5.4.99.20]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.20

Protein Sequence (177 amino acids)

>ABIE51_RS00615 pseudouridine synthase (Lysobacter sp. OAE881)
MLIALNKPYGVLCQFTDRSTPPRRTLAEFGLPPDVYAAGRLDHDSEGLLLLTDDGALAHR
LTDPRHKEPKTYWVQVEGEPSDAQIEALQRGVLLNDGPTRPAKVQRLDAPALWPRDPPVR
FRKTVPDAWLEITISEGRNRQVRRMTAAVGLPTLRLVRVAIGSHRLDDLAPGQWRQL