Protein Info for ABIE51_RS00540 in Lysobacter sp. OAE881

Annotation: NADP-dependent isocitrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 741 TIGR00178: isocitrate dehydrogenase, NADP-dependent" amino acids 4 to 741 (738 residues), 1355.7 bits, see alignment E=0 PF03971: IDH" amino acids 6 to 738 (733 residues), 1316.1 bits, see alignment E=0

Best Hits

Swiss-Prot: 77% identical to IDH_AZOVI: Isocitrate dehydrogenase [NADP] (icd) from Azotobacter vinelandii

KEGG orthology group: K00031, isocitrate dehydrogenase [EC: 1.1.1.42] (inferred from 77% identity to avn:Avin_28310)

MetaCyc: 77% identical to isocitrate dehydrogenase (NADP-dependent) (Azotobacter vinelandii)
Isocitrate dehydrogenase (NADP(+)). [EC: 1.1.1.42]

Predicted SEED Role

"Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42)" (EC 1.1.1.42)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (741 amino acids)

>ABIE51_RS00540 NADP-dependent isocitrate dehydrogenase (Lysobacter sp. OAE881)
MPNTPKILYTLTDEAPFLATQSLLPIIEAYTAPAGIAVETRDISLAGRILSQFPELLKPD
QKIADDLAELGQLATTPDANIIKLPNISASVPQLKAAIAELQQQGYALPDYPEEPKGEQE
NDIKSRYDRVKGSAVNPVLREGNSDRRAPLSVKNYARKHPHRIGAWSADSASHVAHMDAG
DFYASEQSKVIDKAGTVSIELTGADGRRNMLKTNIKVQAGEVIDASLMSAKALSAFVDAQ
IDDALARGVLFSLHLKATMMKVSDPIMFGIVVKRFYRDTLAKHADVLARAGFDANNGIGD
LYARLPSLPPETVAAIEADIAAEYARRPSLAMVNSDKGITNLHVPSDVIVDASMPAMIRD
SGRMWNTDGKLQDTKAIIPDRCYAGIYQAVVDDCKAHGAFDPATMGSVPNVGLMAQKAEE
YGSHDKTFQIPADGVVRVLDDAGHVLMEHTVEAGDIWRMCQTKDAPIQDWVKLAVNRARL
SNTPAVFWLDPQRAHDANLIAKVERYLKDHDTAGLDIRILSPVEAMKLSLSRIRDGKDTI
SVTGNVLRDYLTDLFPIMELGTSAKMLSIVPLMAGGGLFETGAGGSAPKHVQQFVEENYL
RWDSLGEFLALAASLEHLANHTGNARAKVLADTLDEANAKFLDNDKSPSRKVGGIDNRGS
HFYLAMYWARALANQTNDAELATRFGKLADALEANEQKIVDEQIAAQGKPVDIGGYYHPD
MGKLAKAMRPSETFNAALAAL