Protein Info for ABCV34_RS14475 in Castellaniella sp019104865 MT123

Annotation: bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 PF01209: Ubie_methyltran" amino acids 26 to 251 (226 residues), 268.8 bits, see alignment E=1.1e-83 TIGR01934: ubiquinone/menaquinone biosynthesis methyltransferase" amino acids 30 to 250 (221 residues), 253.1 bits, see alignment E=9.1e-80 PF13489: Methyltransf_23" amino acids 65 to 235 (171 residues), 44.2 bits, see alignment E=5.4e-15 PF13847: Methyltransf_31" amino acids 67 to 189 (123 residues), 55.5 bits, see alignment E=1.7e-18 PF13649: Methyltransf_25" amino acids 72 to 167 (96 residues), 73.7 bits, see alignment E=4.8e-24 PF08242: Methyltransf_12" amino acids 73 to 169 (97 residues), 44.1 bits, see alignment E=8.8e-15 PF08241: Methyltransf_11" amino acids 73 to 171 (99 residues), 67.3 bits, see alignment E=4.6e-22

Best Hits

Swiss-Prot: 81% identical to UBIE_BORA1: Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE) from Bordetella avium (strain 197N)

KEGG orthology group: K03183, ubiquinone/menaquinone biosynthesis methyltransferase [EC: 2.1.1.- 2.1.1.163] (inferred from 81% identity to bav:BAV3089)

MetaCyc: 57% identical to bifunctional 2-octaprenyl-6-methoxy-1,4-benzoquinol methylase and demethylmenaquinone methyltransferase (Escherichia coli K-12 substr. MG1655)
2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN [EC: 2.1.1.201]; ADOMET-DMK-METHYLTRANSFER-RXN [EC: 2.1.1.201, 2.1.1.163]

Predicted SEED Role

"Ubiquinone/menaquinone biosynthesis methyltransferase UbiE (EC 2.1.1.-)" in subsystem Menaquinone Biosynthesis via Futalosine or Menaquinone and Phylloquinone Biosynthesis or Ubiquinone Biosynthesis (EC 2.1.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.163 or 2.1.1.201

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (254 amino acids)

>ABCV34_RS14475 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE (Castellaniella sp019104865 MT123)
MIESSHDAGQGPQTHFGFETVRESDKAHRVADVFHSVASRYDLMNDLMSGGLHRIWKAFT
VGRAQIRPGMRVLDIAGGTGDLARAFARRAGPTGQVWLTDINDSMLRVGRDRLLDRGLVT
PVAVCDAECLPFPDRYFDRVSVAFGLRNMTHKDRALAEMHRVLKPGGKLLVLEFSRVARP
LAPVYDWYSFNVLPKMGQLVASDEASYRYLAESIRMHPDQETLADMLRTVGFSRVQYFNM
TVGVVALHEGVRLN