Protein Info for ABCV34_RS13800 in Castellaniella sp019104865 MT123

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 20 to 890 (871 residues), 1329.6 bits, E=0
• PF00330: Aconitase
  • amino acids 76 to 561 (486 residues), 557.2 bits, E=3.5e-171
• PF00694: Aconitase_C
  • amino acids 690 to 820 (131 residues), 142.9 bits, E=7.8e-46

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 68% identity to ahe:Arch_0796)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• mixed acid fermentation (12/16 steps found)
• TCA cycle III (animals) (7/10 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• reductive TCA cycle I (6/11 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (10/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (891 amino acids)

>ABCV34_RS13800 aconitate hydratase AcnA (Castellaniella sp019104865 MT123)
MAYPNSFGARSTLDVGGKPYRIYRLDALKNHGLDPSRLPYGLKILLENLLRTEDGGDVTA
DDIRALASWDPDAQPSREISFTPARVILQDFTGVPAVVDLAAMREAMQALGGKPERINPL
APVELVIDHSVIVDDFGHNDSYRRNVEIEYQRNVERYQFLRWGQTAFDGFRVVPPSTGIV
HQVNLEHLARGVFTRQIDGQTEAYPDTCVGTDSHTPMVNGLGVVAWGVGGIEAEAAMLGQ
PISMLIPRVVGFKLSGALPEGTTATDLVLTITDMLRKHGVVGKFVEFYGPGVASVPLANR
ATIGNMSPEYGSTISIFPIDEETLNYLRFTGRPAEQVALVEAYAKAQGLWHDAAHEPNYS
ERLELDLGTVVPSIAGPKRPQDRILLSESKPSFAKSLAALPGSPKSHPASVHMDGGVDFQ
IDHGAVAIAAITSCTNTSNPAVMVAAGLLAKKAVERGLHRKPWVKTSLAPGSRVVTDYYE
KSGLTKYLDALGFNLVGYGCTTCIGNSGPLIPEISDAVRANDLAVASVLSGNRNFEGRIH
SEVKMNYLMSPPLVVAYALAGSMDFDLYKTPLGQDPQGKDVYLRDIWPTSAEVQDVIRSS
ITADMYTRGYADVFAGNDRWRDLPTPEGDLYRWDDASTYVRNPPYFEGMRRDPDPVGDIH
GARVLAKLGDSVTTDHISPAGSIAKDSPAAQYLQSQGVQPKDFNSYGSRRGNHEVMIRGT
FANVRLRNQIAPDTEGGWTRDFTQDGAPVTTIFDASQNYQKAGIPLVILAGKEYGSGSSR
DWAAKGTVLLGVRAVIAESYERIHRSNLIGMGVLPLQFPEGQNATTLGLRGKETFEITGI
EAFNDGKTPKTVHVKAGDVEFDALVRIDTPSEASYYRHGGILQYAIRQLLG