Protein Info for ABCV34_RS13705 in Castellaniella sp019104865 MT123

Annotation: phenylacetate--CoA ligase PaaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02155: phenylacetate-CoA ligase
  • amino acids 15 to 436 (422 residues), 717.9 bits, E=1.6e-220
• PF00501: AMP-binding
  • amino acids 88 to 293 (206 residues), 60.5 bits, E=1.3e-20
• PF14535: AMP-binding_C_2
  • amino acids 339 to 436 (98 residues), 78.7 bits, E=3.2e-26

Best Hits

Swiss-Prot: 70% identical to PAAK_AZOEV: Phenylacetate-coenzyme A ligase (paaK) from Azoarcus evansii

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 75% identity to tmz:Tmz1t_1509)

MetaCyc: 70% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

• penicillin G and penicillin V biosynthesis (3/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• phenylacetate degradation I (aerobic) (4/9 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (3/9 steps found)
• superpathway of phenylethylamine degradation (4/11 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (5/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Protein Sequence (440 amino acids)

>ABCV34_RS13705 phenylacetate--CoA ligase PaaK (Castellaniella sp019104865 MT123)
MPVKTVKPGDLEAIETASRDEITALQLKRLRETLRHAYDNVPHYRKAFDTRGVHPDDLRT
LDDLRKFPCTTKEDLRRNYPLGMFAVPRERLARLHASSGTTGKPTVVGYTQKDIDTWANL
VARSIRAAGGRRGDTVHVAYGYGLFTGGLGAHYGAERLGCTVIPMSGGQTEKQVQLIMDL
RPDIIMVTPSYSLVIAEEFERQGIAPDDISLKVGIFGAEPWGEGMRSEIERQLGLDAVDI
YGLSEVMGPGVASECIESKDGPVIWEDHFYPEILNPDTGEPVADGEEGELVFTSLTKEAL
PIIRYRTRDLTRLLPPTARSFRRMGRITGRSDDMLIIRGVNVFPTQVEEQILRDPQLNGN
YQILVSRDGHLDNVEVRCELQHDADALSGEQVGQIGRLLQHHIKTNIGISTRVTVMHYGE
LPRTQTGKARRVFDDRPKQH