Protein Info for ABCV34_RS12935 in Castellaniella sp019104865 MT123

Annotation: GDP-mannose 4,6-dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01472: GDP-mannose 4,6-dehydratase
  • amino acids 6 to 344 (339 residues), 600.7 bits, E=4.1e-185
• PF01370: Epimerase
  • amino acids 8 to 255 (248 residues), 261.6 bits, E=1.3e-81
• PF16363: GDP_Man_Dehyd
  • amino acids 9 to 338 (330 residues), 524 bits, E=3.9e-161
• PF02719: Polysacc_synt_2
  • amino acids 9 to 120 (112 residues), 21 bits, E=3.4e-08
• PF04321: RmlD_sub_bind
  • amino acids 9 to 174 (166 residues), 28 bits, E=2.3e-10

Best Hits

Swiss-Prot: 71% identical to GM4D_ECOL6: GDP-mannose 4,6-dehydratase (gmd) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01711, GDPmannose 4,6-dehydratase [EC: 4.2.1.47] (inferred from 87% identity to tbd:Tbd_1776)

MetaCyc: 77% identical to GDP-mannose 4,6-dehydratase (Brucella abortus 2308)
GDP-mannose 4,6-dehydratase. [EC: 4.2.1.47]

Predicted SEED Role

"GDP-mannose 4,6-dehydratase (EC 4.2.1.47)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 4.2.1.47)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
• GDP-6-deoxy-D-talose biosynthesis (1/2 steps found)
• GDP-D-perosamine biosynthesis (1/2 steps found)
• GDP-D-rhamnose biosynthesis (1/2 steps found)
• GDP-L-colitose biosynthesis (3/5 steps found)
• GDP-N-acetyl-α-D-perosamine biosynthesis (1/3 steps found)
• GDP-N-formyl-α-D-perosamine biosynthesis (1/3 steps found)
• GDP-mycosamine biosynthesis (1/3 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (8/14 steps found)
• superpathway of candicidin biosynthesis (3/11 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.47

Protein Sequence (364 amino acids)

>ABCV34_RS12935 GDP-mannose 4,6-dehydratase (Castellaniella sp019104865 MT123)
MTDHTKVALITGITGQDGAYLAEFLLNKGYIVHGIKRRSSLFNTDRIDHLYQDSHEKCVR
FFLHHGDMTDSSSLIRIIQQTQPDEVYNLAAQSHVAVSFEEPEYTANSDALGVLRLLEAI
RILGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYGYWIVVNYREAYGM
YACNGILFNHESPIRGETFVTRKITRAMARIKLGLQDCLFLGNLDAKRDWGHARDYIEMQ
WLMLQQDQPEDFVIATGMQYSVRDFLDATARELGIKISWEGQGVDEKGYDEQGKCIVAVD
PRYFRPTEVETLLGDASKAKQKLGWTPRTSFDELVMEMVREDLRAAERDELVKRHGYKAM
DYKE