Protein Info for ABCV34_RS12365 in Castellaniella sp019104865 MT123

Annotation: bifunctional isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 619 PF20423: AceK_regulatory" amino acids 24 to 343 (320 residues), 388.9 bits, see alignment E=1.6e-120 PF06315: AceK_kinase" amino acids 344 to 598 (255 residues), 398.1 bits, see alignment E=1.8e-123

Best Hits

Swiss-Prot: 73% identical to ACEK_BORBR: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 76% identity to put:PT7_3028)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (619 amino acids)

>ABCV34_RS12365 bifunctional isocitrate dehydrogenase kinase/phosphatase (Castellaniella sp019104865 MT123)
MRDDGDHRRIEPVTPRGQSPEAVARMILAGFDRHYSLFRYSAQRAKALFESGDWHGIQQL
SRERIEYYDTRVRECATALGQALRGTTPAQTAPDTALTPEQLEFWQAAKTSYIGLLASHL
QPECAETFFNSVSCRILHRNYFNNDFLFVRPAVATEYIDGALSTFRSYYPLTEGLLPCLV
RLVADFGLAAPYQDLPADTRRLARLGLRHLRHTLPRGLGKRLAPDCQLQVLNTLFFRNKG
AYIVGRLINQTAIHPFAIALLHTPSGHIQLDALLHTSDDLSTLFSFTRAYFLVDMEAPAA
YVDFLSSLFPRKPKAEIYTTIGLQKQGKTLFYRDFLHHLAHSRDSFDYAPGIQGMVMTVF
TLPSYPYVFKLIRDHIAKDGMDEATVRRKYQMVKKHDRVGRMADTWEYSLVALPRSRFSP
RLLAGLRAEVPSLLEETDDLVVLKHVYIERRMTPLNIFLASASDTALEAAVRQYGQAIRE
LAAANIFPGDMLYKNFGVTRLGRVVFYDYDEIQRMTEMQFRRIPPAPNEEAELSGEPWYP
VGANDVFPEEFRHFLLGDDRVRAAFLKHHAALLDAQWWQDCRTRASQGRIEDIFPYDQAR
RLHFPEPAASTPNAALPTL