Protein Info for ABCV34_RS10410 in Castellaniella sp019104865 MT123

Annotation: poly-beta-1,6-N-acetyl-D-glucosamine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 34 to 54 (21 residues), see Phobius details amino acids 323 to 349 (27 residues), see Phobius details amino acids 363 to 384 (22 residues), see Phobius details amino acids 396 to 418 (23 residues), see Phobius details TIGR03937: poly-beta-1,6 N-acetyl-D-glucosamine synthase" amino acids 31 to 439 (409 residues), 630.1 bits, see alignment E=8.2e-194 PF13641: Glyco_tranf_2_3" amino acids 77 to 298 (222 residues), 113.8 bits, see alignment E=2.4e-36 PF00535: Glycos_transf_2" amino acids 78 to 245 (168 residues), 117 bits, see alignment E=1.7e-37 PF13506: Glyco_transf_21" amino acids 143 to 297 (155 residues), 58.4 bits, see alignment E=1.4e-19 PF13632: Glyco_trans_2_3" amino acids 158 to 363 (206 residues), 86.6 bits, see alignment E=4.2e-28

Best Hits

Swiss-Prot: 60% identical to PGAC_ECOLI: Poly-beta-1,6-N-acetyl-D-glucosamine synthase (pgaC) from Escherichia coli (strain K12)

KEGG orthology group: K11936, biofilm PGA synthesis N-glycosyltransferase PgaC [EC: 2.4.-.-] (inferred from 74% identity to axy:AXYL_02332)

MetaCyc: 60% identical to poly-N-acetyl-D-glucosamine synthase subunit PgaC (Escherichia coli K-12 substr. MG1655)
2.4.1.M63 [EC: 2.4.1.M63]; TRANS-RXN0-549 [EC: 2.4.1.M63]

Predicted SEED Role

"Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-)" (EC 2.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.-.-

Use Curated BLAST to search for 2.4.-.- or 2.4.1.M63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (445 amino acids)

>ABCV34_RS10410 poly-beta-1,6-N-acetyl-D-glucosamine synthase (Castellaniella sp019104865 MT123)
MTDRLLAFLILCLVLGAPFGAAMLWTGDIILDFVFFYPLFMSGLWMFGGLYYWLHWERHW
PWGPDVAPPELKGGPLVSILIPCYNEAEHGAETILAALDQRYLRVEVIAINDGSTDNTGA
MLDELAVVHDRLRVVHLAQNQGKAMALRMGALAARSDYLICIDGDAILDRDAVNYLVAPL
LEHPRVGAVTGNPRVRTRSTLIGRVQVGEFSSIVGLIKRTQRVYGQVFTVSGVVAAFRRS
ALDRVGYWSLDMVTEDIDISWKLQRDHWSIFFEPRALCWILMPETLHGLWKQRLRWAQGG
AEVFLKNVPNIWSWHNRRLWPMVLEYCISAIWAFALILSIILWAIGLFVTLPHHIHVETL
LPPAFTGMVLAMACLLQFCLSVFIDHRYERNLSHSLYWVIWYPLAYWMVNLLTTLWSFPK
VMLKHHRRCRARWESPDRGIKAPTS