Protein Info for ABCV34_RS08455 in Castellaniella sp019104865 MT123

Annotation: polyhydroxyalkanoate depolymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 TIGR01849: polyhydroxyalkanoate depolymerase, intracellular" amino acids 2 to 403 (402 residues), 597.2 bits, see alignment E=6.7e-184 PF06850: PHB_depo_C" amino acids 203 to 404 (202 residues), 311.5 bits, see alignment E=1.1e-97

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 77% identity to bpt:Bpet1727)

Predicted SEED Role

"Intracellular PHB depolymerase (EC 3.1.1.-)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.-)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.- or 3.1.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (405 amino acids)

>ABCV34_RS08455 polyhydroxyalkanoate depolymerase (Castellaniella sp019104865 MT123)
MLYDLHELQRSLLAPFTAFTETGSQLFSNPYSPLAYTPLSRQFAAAYELAHRVGKDYQKP
AWNLPFTTIDGRETAVHEDIALAQPFCHLVHFRRETTRQDPRVLLVAPLSGHHATLLRDT
VRALLPSHDVYVTDWIDARMVPIADGTFGLDDYVHYIQAFLVHLGPDTHVISVCQPTVPV
LAAVSLLASTDAPCQPRTMIMMGGPIDTRRSPTQVNRLATTKPYAWFEDNLIHRVPPRYV
GAGRLVYPGFLQHAGFVAMNPDRHVKSHYDFYLDLLRGDDSDAQAHRRFYDEYNAVLDMP
AQFYLDTIRTVFQEFRLPRGTWTIDGQLVEPTAIRKTALLTIEGELDDISGEGQTHAAHD
LCASLPDGMREAHTAAGCGHYGIFSGRRWRAQICPKISSFIRAHA