Protein Info for ABCV34_RS06890 in Castellaniella sp019104865 MT123

Annotation: nitrite reductase large subunit NirB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 PF07992: Pyr_redox_2" amino acids 18 to 297 (280 residues), 171 bits, see alignment E=1.4e-53 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 19 to 810 (792 residues), 1066.4 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 159 to 224 (66 residues), 47.4 bits, see alignment 7.7e-16 PF18267: Rubredoxin_C" amino acids 330 to 393 (64 residues), 40.2 bits, see alignment 9.3e-14 PF04324: Fer2_BFD" amino acids 433 to 480 (48 residues), 42.7 bits, see alignment 2e-14 PF03460: NIR_SIR_ferr" amino acids 569 to 630 (62 residues), 48 bits, see alignment 2.9e-16 PF01077: NIR_SIR" amino acids 641 to 762 (122 residues), 73.5 bits, see alignment E=4.8e-24 PF13806: Rieske_2" amino acids 866 to 968 (103 residues), 103.5 bits, see alignment E=2e-33 TIGR02378: nitrite reductase [NAD(P)H], small subunit" amino acids 866 to 968 (103 residues), 103.7 bits, see alignment E=5.6e-34

Best Hits

Swiss-Prot: 57% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 61% identity to azo:azo1175)

MetaCyc: 57% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (987 amino acids)

>ABCV34_RS06890 nitrite reductase large subunit NirB (Castellaniella sp019104865 MT123)
MNPHASPTTTTGSVPRAKLVIIGHGMVGHRLLEELARDAHGYDITVFCSEPALAYDRVHL
SQCFDGAGPAELALADEAFFNEHGITVLRGQHAAWVDRKKRRVHLDKDQSYPYDKLVFAT
GSVPFIPPIEGADRPGCLPYRTLQDVKAIRESAAGGRRGVVIGGGLLGLEAARALQGLKL
ETHVVEFSPWLMTTQLDEPGGRLLRKKIEALGIHVHPSKMTASIGNGLCSRHRLRFSDGD
FLESDLVVFSAGIRPCDRLANGCGLVIGERGGIVIDEHCRTSDPDIHAIGEVASWHGGTF
GLVAPGYEMARVCAAHLTGRDAPVFQGTLPGTKLKLLGVDVASIGDAQGRTPDSVSCVFE
DTLAGVYKKLVLDPTGKRLLGAVLVGDTEAFGVLDQYFRTGMNLPGQPATLIAPPAGDTP
ALGVDTLPDSAQICSCNQVSKGDLCGAIAAGCTTLGDLKSRTRAGATCGGCVPLVKQVLE
SELQRRGIAVDHSLCEHFAYSRQELYHLIRVNQIRDFNTLITRHGKGRGCDICKPTAASI
FASCWNDYVLSAPLAPLQDSNDAYLGNLQKDGTYSVVPRIPGGEITPDKLIVIGEVARQY
DLYTKITGGQRIDLFGARLEQLPEIWKRLIDAGFESGHAYGKSLRTAKSCVGSTWCRYGV
QDSVGLAIRLEDRYRGLRAPHKIKMAASGCTRECAEAQSKDVGVIATEKGWNLYVCGNGG
MRPRHAELLVAGLDDEALFRTIDRFLMFYIRTADKLQRTAVWRDSLEGGLDYLKDVVLHD
SLGIAAELEAQMQAVVKSYECEWTSTLKDPKALRRFVPAVNSDAPDEHIIHRRERGQPRP
ATRAEAAQDAANRPVHAPMVRPESAEWQDVCHVDDLIPDAGILVRAHGTQIAVFHTEAGL
FAIDHVDPFSGAAILARGIVGDLQGHTVVASPMYKQHFRLDTGECLEDGTMSVRCWPLHA
AKNGRISIGRTAPTALKETAHALEPEV