Protein Info for ABCV34_RS06190 in Castellaniella sp019104865 MT123

Annotation: NADP-specific glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 56 to 183 (128 residues), 169.2 bits, E=3.4e-54
• PF00208: ELFV_dehydrog
  • amino acids 201 to 444 (244 residues), 306.9 bits, E=1.2e-95

Best Hits

Swiss-Prot: 73% identical to DHE4_SALTI: NADP-specific glutamate dehydrogenase (gdhA) from Salmonella typhi

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 85% identity to put:PT7_1431)

MetaCyc: 73% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Arginine and Ornithine Degradation or Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Proline Synthesis (EC 1.4.1.4)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-glutamate biosynthesis III (1/1 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.4

Use Curated BLAST to search for 1.4.1.4

Protein Sequence (446 amino acids)

>ABCV34_RS06190 NADP-specific glutamate dehydrogenase (Castellaniella sp019104865 MT123)
MSLPSVEQFLSQLQARDPHQPEFMQAVKEVMHSLWPFIQKNPRYASQALLERIVEPERAF
QFRICWMDDTGHTRVNRGFRIQHSSAIGPYKGGMRFHPSVNLSILKFLAFEQTFKNALTT
LPMGGGKGGSDFDPKGKSDAEVMRFCQALIIELYRHLGPDTDVPAGDIGVGAREVGFMAG
MMKKLSNQGACVFTGKGLSFGGSLIRPEATGYGAVYFAEEMLKHAGQSLDGYTVSVSGSG
NVAQYTIEKCMQLGARVITVSDSHGCVVDPAGFTPEKLAALIELKNEKRGRVEEYARQFG
LTYEAGKRPWHVPVDVALPCATQNELEIEDARTLIANGVRCVAEGANMPSTIEAAEAFIA
AGVLYAPGKASNAGGVAVSGLEMSQNAQRLHWTRDEVDAKLHAIMRDIHQNCVHHGSEAG
KVNYLNGANIAGFVKVADAMLAQGVY