Protein Info for ABCV34_RS04805 in Castellaniella sp019104865 MT123

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 648 PF02824: TGS" amino acids 2 to 66 (65 residues), 44.8 bits, see alignment E=2.1e-15 TIGR00418: threonine--tRNA ligase" amino acids 77 to 635 (559 residues), 701.2 bits, see alignment E=6.6e-215 PF07973: tRNA_SAD" amino acids 174 to 223 (50 residues), 59.6 bits, see alignment 4.7e-20 PF00587: tRNA-synt_2b" amino acids 322 to 533 (212 residues), 166 bits, see alignment E=2e-52 PF03129: HGTP_anticodon" amino acids 546 to 634 (89 residues), 68 bits, see alignment E=1.3e-22

Best Hits

Swiss-Prot: 82% identical to SYT_BORPA: Threonine--tRNA ligase (thrS) from Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 84% identity to put:PT7_1277)

MetaCyc: 56% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (648 amino acids)

>ABCV34_RS04805 threonine--tRNA ligase (Castellaniella sp019104865 MT123)
MVRITLPDGSQREFDHPVTVHDVAHSIGTGLGKAALGGKITVDGQPARLVDTSHVIDRDA
ALSIITDKDPEGLDLIRHSTAHLLAYAVKSLFPDAQVTIGPVIDNGFYYDFSYKRPFTPE
DLQAIEKKMAELAKRDETVVREEWDRDEAVKFFRGIGETYKAEIIASIPANEKISLYREG
GFVDLCRGPHVPSTGRLKVFKLMKVAGAYWRGDSKNEMLQRIYGTAWARQADQDAYLHML
EQAELRDHRRIGRELDLFHFQDEAPGLIFWHPKGWTIWQQVEQYMRRVYQDNGYQEVKAP
QILDLSLWKKTGHWDNYKENMFTTESENRVYGLKPMNCPGHVQIFNAGLHSYRELPLRYG
EFGQCHRNEPSGSLHGMMRVRGFTQDDGHIFCTEEQLQEECAAFTRLLQQVYADFGFTEI
LYKVATRPDKRIGADDVWDKAEKALMDSLDLSHCDYQVSEGEGAFYGPKIEYTLKDAIGR
HWQCGTIQVDFSMPARLGAEYVDAQDQRRTPVMLHRAILGSFERFIGMLIENHAGAMPAW
LAPHHAVVCCISDSSADYADEVSKTLVKQGFRVLSDLRGEKITRKIREHSLQKIPYILVI
GDKERDSGAVAVRGRGNLDLGTMPVADFAARLRQDIETRHQEGDPAQA