Protein Info for ABCV34_RS04150 in Castellaniella sp019104865 MT123

Annotation: dihydrolipoyl dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 5 to 76 (72 residues), 71 bits, E=2.3e-23
• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 131 to 587 (457 residues), 486.1 bits, E=5e-150
• PF01134: GIDA
  • amino acids 132 to 194 (63 residues), 29.5 bits, E=1.7e-10
• PF07992: Pyr_redox_2
  • amino acids 132 to 453 (322 residues), 204.6 bits, E=9.7e-64
• PF00890: FAD_binding_2
  • amino acids 132 to 166 (35 residues), 23.8 bits, see alignment (E = 1e-08)
• PF12831: FAD_oxidored
  • amino acids 132 to 169 (38 residues), 39.4 bits, 2.1e-13
• PF00070: Pyr_redox
  • amino acids 301 to 376 (76 residues), 44.8 bits, E=6.1e-15
• PF02852: Pyr_redox_dim
  • amino acids 472 to 580 (109 residues), 126 bits, E=3.1e-40

Best Hits

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 77% identity to axy:AXYL_02723)

MetaCyc: 74% identical to dihydrolipoamide dehydrogenase (Advenella mimigardefordensis DPN7)
Dihydrolipoyl dehydrogenase. [EC: 1.8.1.4]; 1.8.1.- [EC: 1.8.1.4]

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• 3,3'-disulfanediyldipropannoate degradation (2/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.8.1.4

Protein Sequence (597 amino acids)

>ABCV34_RS04150 dihydrolipoyl dehydrogenase (Castellaniella sp019104865 MT123)
MSIKDVNVPDIGDFDQVDVIEILVAVGDTIQPEQSLITVESDKASMEIPADQGGVVKEIL
VKIGDKVSQGMAILRIDSTDSAAVSAPAPAAPAPAAATNAAPAQPAVAVAAPAVQAGPAP
AQFAGTADADCDVLVLGAGPGGYSAAFRAADLGLKVVLVERYTTLGGVCLNVGCIPSKAL
LHTAAVLEEAKSLSAHGITFGEPKIDLDAVRGYKDSVVGKLTGGLAGMAKARKVTVIQGV
GTFADPHHLTVTKPDGGQTTVKFASAIIAAGSQSVKLPFLPDDPRIVDSTGALALKSIPK
RMLIVGGGIIGLEMGTVYSALGARLDVVEMLPTLMTGADRDLVKVWDKLNAQRFDHVMLK
TKTVAAEAKDDGIWVTFEGEAAPKEPQRYDLVLQAVGRSPNGKKIGAENAGVIVGDRGFI
AVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAIAGEKAFFDARVIPSVAYTDP
EVAWVGLTEEEAKKQGVALKKGLFPWQASGRAIANGRDEGFTKLLFDAETGRVLGGGIVG
THAGDLISEVALAIEMGADSVDIGKTIHPHPTLGESIGMAAEVADGHCTDVPPTKKK