Protein Info for ABCV34_RS03815 in Castellaniella sp019104865 MT123

Annotation: haloacid dehalogenase type II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 227 TIGR01428: haloacid dehalogenase, type II" amino acids 6 to 199 (194 residues), 207.4 bits, see alignment E=2.3e-65 PF00702: Hydrolase" amino acids 7 to 187 (181 residues), 80.7 bits, see alignment E=1.9e-26 TIGR01493: HAD hydrolase, family IA, variant 2" amino acids 7 to 184 (178 residues), 117.3 bits, see alignment E=7.9e-38 PF13419: HAD_2" amino acids 91 to 192 (102 residues), 40.8 bits, see alignment E=2.7e-14

Best Hits

Swiss-Prot: 48% identical to HAD4_BURCE: (S)-2-haloacid dehalogenase 4A (hdl) from Burkholderia cepacia

KEGG orthology group: K01564, [EC: 3.8.1.-] K07025, putative hydrolase of the HAD superfamily (inferred from 53% identity to sme:SMc00103)

MetaCyc: 44% identical to S-2-haloacid dehalogenase (Burkholderia sp. WS)
(S)-2-haloacid dehalogenase. [EC: 3.8.1.2]; 2-haloacid dehalogenase (configuration-inverting). [EC: 3.8.1.2, 3.8.1.10]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.8.1.- or 3.8.1.10 or 3.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (227 amino acids)

>ABCV34_RS03815 haloacid dehalogenase type II (Castellaniella sp019104865 MT123)
MPLPLIAVFDAYGTLFDVHSAVRRVAGSAGANAGRVSALWRDKQLEYSWTRSLMGRYADF
WQVTEDALDYALSTCKVDDTKLRGALLTAYRTLDPYPDVRAALTGYHDLGVRTAVFSNAT
PSMLHDALVSAGLDEIVDTTFSTDTLRRYKPAPEVYAQACKAFSVKPETIVFHSSNAWDA
AGAASFGWRTFWINRTHGAPEYTWADFNECSSLDAVLARVSAVKQTP