Protein Info for ABCV34_RS03095 in Castellaniella sp019104865 MT123

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 754 PF13328: HD_4" amino acids 68 to 217 (150 residues), 171.6 bits, see alignment E=3.2e-54 TIGR00691: RelA/SpoT family protein" amino acids 68 to 749 (682 residues), 733.1 bits, see alignment E=1.6e-224 PF01966: HD" amino acids 87 to 186 (100 residues), 43.9 bits, see alignment E=8.3e-15 PF04607: RelA_SpoT" amino acids 277 to 387 (111 residues), 130.8 bits, see alignment E=8.3e-42 PF02824: TGS" amino acids 430 to 489 (60 residues), 69.1 bits, see alignment 8.1e-23 PF19296: RelA_AH_RIS" amino acids 502 to 589 (88 residues), 28.8 bits, see alignment E=3.8e-10 PF13291: ACT_4" amino acids 676 to 751 (76 residues), 52.8 bits, see alignment E=1.6e-17

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 83% identity to put:PT7_0991)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (754 amino acids)

>ABCV34_RS03095 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Castellaniella sp019104865 MT123)
MAFSGFRGASSGLLAVLRKGPRLRRRTPPNATAALPVSAPSPIASLAGLQQIIAGYLSPR
DIERVREAYRFADNAHLGQFRESGEPYISHPIAVTEICAGWKLDADSLMAALLHDVIEDQ
GISKQELAEKFGGEVADIVDGLTKLERLEYATKAEQQAESFRKMLLAMARDVRVILIKIA
DRLHNMRTLDAVAPEKRRRVARETLDIYTPIAHRLGLNALFRELQDRCFKAIYPNRYDVL
HKAMMAARGNRREVISKIAETVRAALPAAGLDAEVNGREKSLYGIYKKMVEQKKSFSDVL
DIYGFRVIVHTLPECYLAMGTLHQLYRPVPGKFKDYIAIPKVNGYQSLHTTLIGPYGTPI
EFQFRTRDMDHVAEEGVASHWLYKEQGTSLNDLQKRTHQWLQSLLDIQSQTGDSGEFLEH
VKVDLFPDAVYVFTPQGKILSLPRGATPVDFAYAIHTDVGNHAVACKINGDFSPLRTELH
SGDAVEIMTSSASRPSAQWLNYVRTGRARSEIRHYLRTERYEESIAFGRRLLNQAFDQLG
ITYPKDDDPQWDRLAKGSGGASRTEILADIGLGKRLAAVVARRFAPENAMLETTAMAVDE
FNSVTSAPIVIHGNEGQAVQLAHCCGPLPGDAIIGGIRLGHGLVVHMADCPVALRQQAKE
PERWIPVVWDAKTARHLTTRLDITVLNERGVLGRLAAEVTEADSNIIQLTMPDEAAATAL
LHLTVQVDSRKHLAQVIRAIRHVPQVQKIVRVKG