Protein Info for ABCV34_RS02640 in Castellaniella sp019104865 MT123

Annotation: ribosome biogenesis GTPase Der

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03594: ribosome-associated GTPase EngA
  • amino acids 5 to 451 (447 residues), 560.4 bits, E=4.4e-172
• TIGR00231: small GTP-binding protein domain
  • amino acids 6 to 159 (154 residues), 57.6 bits, E=2e-19
  • amino acids 195 to 362 (168 residues), 90 bits, E=2.2e-29
• PF02421: FeoB_N
  • amino acids 7 to 128 (122 residues), 39.7 bits, E=1.5e-13
  • amino acids 197 to 360 (164 residues), 45.4 bits, E=2.6e-15
• PF01926: MMR_HSR1
  • amino acids 7 to 122 (116 residues), 93.7 bits, E=3.3e-30
  • amino acids 198 to 315 (118 residues), 92 bits, E=1.1e-29
• PF00009: GTP_EFTU
  • amino acids 228 to 362 (135 residues), 43.6 bits, E=1e-14
• PF14714: KH_dom-like
  • amino acids 371 to 451 (81 residues), 98.7 bits, E=7.6e-32

Best Hits

Swiss-Prot: 75% identical to DER_BORPD: GTPase Der (der) from Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)

KEGG orthology group: K03977, GTP-binding protein (inferred from 75% identity to bpt:Bpet2023)

MetaCyc: 50% identical to 50S ribosomal subunit stability factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (463 amino acids)

>ABCV34_RS02640 ribosome biogenesis GTPase Der (Castellaniella sp019104865 MT123)
MHFKPVVVLVGRTNVGKSTLFNRLTRSRAALVANISGLTRDRHYGEGRMGDHPFIVVDTG
GFEPVATQGILVEMARQTAQAIAEADVVLFLVDGREGINAHDHEIARLLRKAGQRVLLAV
NKAEGMRHHADLAQFYELGLGDPHPISAAHGDGVSALVDEALGPLLVPEPSHSLDADPLA
ITEEAEDEADVPDHRIKLAIAGRPNVGKSTLINTLVGEERVIAYDLPGTTRDAIEIEFER
NGRRYTLIDTAGLRRRGKVFETVEKFSVIKTLQAIEASNVVLLLIDAQAGISDQDAHIAG
FATEAGRALVVGLNKWDAVDSEQREWVQREYERKLRFLSFARVLHLSALKGQGVGPVLKA
VEAAHAAAFAKLSTPKLTRTLQAAIEQQPPPRKGIFRPKMRYAHQGGQNPPRIIIHGNAL
DDVPDSYRRYLENQFREAFNLAGTPLSIEFKSSHNPYLKDANP