Protein Info for ABCV34_RS02265 in Castellaniella sp019104865 MT123

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 PF00772: DnaB" amino acids 15 to 116 (102 residues), 113.1 bits, see alignment E=1.2e-36 TIGR00665: replicative DNA helicase" amino acids 15 to 450 (436 residues), 579.8 bits, see alignment E=1.8e-178 PF03796: DnaB_C" amino acids 195 to 449 (255 residues), 368.8 bits, see alignment E=2.6e-114 PF13481: AAA_25" amino acids 208 to 372 (165 residues), 48.5 bits, see alignment E=1.7e-16

Best Hits

Swiss-Prot: 56% identical to DNAB_ECOL6: Replicative DNA helicase (dnaB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 90% identity to put:PT7_0469)

MetaCyc: 56% identical to replicative DNA helicase (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>ABCV34_RS02265 replicative DNA helicase (Castellaniella sp019104865 MT123)
MEIEADSQLDYLRVPPHSIEAEQSVLGGLLLDNAAFDRVADVLTEDDFYRFDHRLIWQHI
TRLVGLARPADVITVHESLTTAGKADDSGGLPYLNALAHNTPSAANIRRYAEIVRERSML
RKLVSIADEISSAAFNPQGKEARQILDEAESRVFQIAQEGARGMAGFQEIRPLLAQVVER
IDELYHREGDSDVTGVPTGFNDLDRMTSGLQPGDLVIVAGRPSMGKTSFSMNIGEHVAIE
QGLPVAVFSMEMGAVQLAMRMIGSVGMLDQHRMRTGKLNQDDWPRLTHAVQKVEQAQIYI
DETPALTSMEVRARARRLSRQCGQLGMIIIDYIQLMSGSGGENRATEISEISRSLKSLAK
ELNCPLVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVIIFIYRDEVYNPDSPDKGT
AEIIIGKQRNGPIGTVRLTFQGSSTRFLNFSGNPGF