Protein Info for ABCV34_RS01740 in Castellaniella sp019104865 MT123

Annotation: tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 PF00579: tRNA-synt_1b" amino acids 13 to 295 (283 residues), 170.9 bits, see alignment E=2e-54 TIGR00233: tryptophan--tRNA ligase" amino acids 14 to 344 (331 residues), 293.6 bits, see alignment E=1e-91

Best Hits

Swiss-Prot: 69% identical to SYW_BORPA: Tryptophan--tRNA ligase (trpS) from Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 76% identity to bpt:Bpet1839)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (453 amino acids)

>ABCV34_RS01740 tryptophan--tRNA ligase (Castellaniella sp019104865 MT123)
MNTKTSVNTPGVSRVLTGITTSGTPHLGNYAGAIRPAIQSSTEPGVDAFFFLADFHALIK
CADPARVAQSRMEIAATWIAAGLDTDRVTFYRQSDVPEIPELCWMLTCVTAKGLMNRAHA
YKASVDVNLAKGEEPDDGITMGLFSYPVLMAADILMFNAHRVPVGRDQIQHLEMTRDIAQ
RFNHQYGGGRDLFVLPEVQIDEAVATLPGLDGRKMSKSYNNTIPLFEGGAKALKSAVAQI
VTDSRLPGEPKDADNSHLFTLYRAFATHEQAIEFRKSLEDGLGWGDAKQALCERIEAQIG
PMRAHYADLMAHPERIEDILRAGAEKARRTAVPFMMRLREAVGLRQPASTAGGKAAGTGK
GAKAGQARLVSFRDEDGRFRQRLFAADGTELLLSEPFGDPKVLNALGRSFAGRDLSSLVA
GTDGLVSLESDGLCWARCPADRVNAVRMALAQV