Protein Info for ABCV34_RS00605 in Castellaniella sp019104865 MT123

Annotation: benzoate-CoA ligase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 transmembrane" amino acids 73 to 87 (15 residues), see Phobius details amino acids 219 to 241 (23 residues), see Phobius details TIGR02262: benzoate-CoA ligase family" amino acids 7 to 513 (507 residues), 745.9 bits, see alignment E=1.1e-228 PF00501: AMP-binding" amino acids 21 to 377 (357 residues), 276.2 bits, see alignment E=3.9e-86 PF13193: AMP-binding_C" amino acids 427 to 505 (79 residues), 68.8 bits, see alignment E=6.4e-23

Best Hits

KEGG orthology group: K04110, benzoate-CoA ligase [EC: 6.2.1.25] (inferred from 69% identity to axy:AXYL_05035)

MetaCyc: 57% identical to benzoate-CoA ligase subunit (Aromatoleum evansii)
Anthranilate--CoA ligase. [EC: 6.2.1.32]; Benzoate--CoA ligase. [EC: 6.2.1.32, 6.2.1.25]

Predicted SEED Role

"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.25 or 6.2.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (520 amino acids)

>ABCV34_RS00605 benzoate-CoA ligase family protein (Castellaniella sp019104865 MT123)
MAPCPEQLNFARHLFDLNAGRATKTAYLDGQRSLSYGELEQRSIRFAGLLKQLGLHREER
ILLLMHDTVDWPVCFLGSLLAGVVPIAANTLLTADDYAYILQNSRCQAAFVSQALLPVLQ
RAMLKVSHEVAHIVVAQPELPLSEGMIDFEAALAQAPAPTPVDTQTDETAFWLYSSGSTG
LPKAVVHTHGNLWYTAELYGKPILGVRESDTVFSAAKMFFAYGLGNALTFPLSVGATVLL
LSGRPTADAVFEHLTRHRPTIFCGVPTLYASMLSSPQLPSKEQLTLRVCASAGEALPKDL
GDRFTEHFGCYILDGIGSTEMLHIFLTNQPGDIHYGTTGKPVPGYEVELRDEAGNPTPVG
QIGDLYIKGPSAALMYWHNRKKSHDTFLGEWLKSGDKYTVDAQGYYTYAGRSDDMIKVSG
QYVSPAEVEGTLIRHPLVLEAAVIGAPDHEGLIKTIAYVVLRPGTTHSEAIELELKTFVK
SNLAPHKYPRIIIFLDDLPKTATGKIQRFRLRQQHESVPA