Protein Info for ABCV34_RS00250 in Castellaniella sp019104865 MT123

Annotation: NADH-quinone oxidoreductase subunit NuoF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF01512: Complex1_51K" amino acids 145 to 314 (170 residues), 144.7 bits, see alignment E=2.3e-46 PF10589: NADH_4Fe-4S" amino acids 428 to 508 (81 residues), 96.5 bits, see alignment E=7.1e-32

Best Hits

KEGG orthology group: K00124, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 74% identity to bbr:BB1322)

Predicted SEED Role

"NAD-dependent formate dehydrogenase beta subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (519 amino acids)

>ABCV34_RS00250 NADH-quinone oxidoreductase subunit NuoF (Castellaniella sp019104865 MT123)
MDKTLYIPLDSAALAMGANALADAFHAEAARRNIPLRIVRNGSRGLLWLEPLVEIDTPAG
RVAYGPVTASDVPSILDAACTDGASHPLCHGLTESIPFLRHQERLTFARVGITDPLSIAD
YEAHGGYAGLRRALDMTPDAIVACITESGLRGRGGAAFPTGIKWRTVLQTPAAQKYIVCN
ADEGDSGTFSDRMLMEGDPFSLIEGMTIAGLAVGATRGYVYVRSEYPQAEHILGLALRAA
RDADWLGNDIQGSGQSFDIELRRGAGAYICGEETSLLESLEGRRGLVRAKPPLPAIRGLF
GCPTVINNVISLATAPFILAQGAQAYRDFGMGKSRGTLPFQLAGNIRQGGLVEKAFGLTL
RELLFDYGGGSASGRPLRAIQVGGPLGAYLPESQWDTPLDYEAFLAVSAMLGHGGVVAFD
DTVDMAAMAEYAMEFCAIESCGKCTPCRIGSVRGTEIIRKIRQDQDRESQAELLQDLCDT
MLAGSLCALGGMTPYPVQSALRHFPEDFGLPRQPVPQTA