Protein Info for DvMF_3145 in Desulfovibrio vulgaris Miyazaki F

Annotation: tRNA (uracil-5-)-methyltransferase Gid (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00070: Pyr_redox" amino acids 7 to 40 (34 residues), 22.1 bits, see alignment 2.7e-08 TIGR00137: tRNA:m(5)U-54 methyltransferase" amino acids 8 to 436 (429 residues), 561.7 bits, see alignment E=5.1e-173 PF13450: NAD_binding_8" amino acids 9 to 38 (30 residues), 23 bits, see alignment (E = 1.2e-08) PF01134: GIDA" amino acids 9 to 374 (366 residues), 310.3 bits, see alignment E=3.2e-96

Best Hits

Swiss-Prot: 77% identical to TRMFO_DESVV: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (trmFO) from Desulfovibrio vulgaris subsp. vulgaris (strain DP4)

KEGG orthology group: K04094, glucose inhibited division protein Gid (inferred from 100% identity to dvm:DvMF_3145)

Predicted SEED Role

"tRNA:m(5)U-54 MTase gid" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DND9 at UniProt or InterPro

Protein Sequence (445 amino acids)

>DvMF_3145 tRNA (uracil-5-)-methyltransferase Gid (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MTALVTAAIAGGGLAGCECARRLARAGVAVTLYEMKPQAFSPAHASPDLAELVCSNSLRS
DDIASGVGLLKQEMRELDSLVMQVAEATRVPAGKALAVDRELFARRMTEAMEAEPNITVV
RREITSIHAPELAAADVAVVSAGPLASEPLAASLAEVVGSRHLYFYDAIAPIIAAESIDT
SIAFQGSRYDEGEGDYLNCPMNREEYEAFHAALLAAEKAPTHEFEKEVHFEGCMPIEALA
ERGERTLVFGPFKPVGFTDPRTGQRPYAIVQLRAENLNRTAYNLVGCQTKLKYGEQERVF
RMIPGLANAEFVRHGSMHRNTYVNAPLVLADDLALRPHRHVHLAGQITGVEGYVESAACG
LWLGMLLAARALGRDLPAPPVDTALGALLHHLRTPAKNFQPSNVNFGLMPELTQRAKKRD
RKALYAERARVSFAAWLEGVPGLEK