Protein Info for DvMF_2717 in Desulfovibrio vulgaris Miyazaki F

Annotation: GTP-binding protein LepA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 PF00009: GTP_EFTU" amino acids 6 to 184 (179 residues), 190 bits, see alignment E=9.7e-60 TIGR01393: elongation factor 4" amino acids 6 to 598 (593 residues), 1006.1 bits, see alignment E=4.7e-307 TIGR00231: small GTP-binding protein domain" amino acids 9 to 178 (170 residues), 70.7 bits, see alignment E=1.3e-23 PF03144: GTP_EFTU_D2" amino acids 208 to 278 (71 residues), 43.6 bits, see alignment E=9.7e-15 PF14492: EFG_III" amino acids 295 to 367 (73 residues), 31.1 bits, see alignment E=5.9e-11 PF00679: EFG_C" amino acids 402 to 488 (87 residues), 87.6 bits, see alignment E=1.4e-28 PF06421: LepA_C" amino acids 491 to 596 (106 residues), 170.3 bits, see alignment E=3.3e-54

Best Hits

Swiss-Prot: 100% identical to LEPA_DESVM: Elongation factor 4 (lepA) from Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 100% identity to dvm:DvMF_2717)

MetaCyc: 62% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DIZ5 at UniProt or InterPro

Protein Sequence (601 amino acids)

>DvMF_2717 GTP-binding protein LepA (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MPRQEHIRNFSIIAHIDHGKSTLADRILEVTGLVSDREKRDQYLDRMELERERGITIKAQ
SVRIPYTAKDGRKYVLNLIDTPGHVDFNYEVSRSLAACEGALLVVDASQGVEAQTLANVY
LALDHNLEVIPVLNKVDLPSADADRVKHEIEESIGLDCSDAVAVSAKTGLNVDKVLEAIV
ERLPAPEGNLNAPLKALIFDSWYDSYQGVVVLFRIVDGVLRKGDRIKLFATERSYEVIRL
GVFSPEIVDMTELGAGEVGFLCANIKELGDAKVGDTITHVDRPAEVPVEGFKEVQPMVFC
GLYPTDSADYEQLKYALEKLQLNDAAFSYEPETSQALGFGYRCGFLGLLHMEIIQERLER
EFQVELIATAPSVIYKIDTVDGKKSDIDNPSKLPDPTKIAALYEPYVRMDIHVPNDYVGN
VLKLCEEKRGIQKNMGYIASNRVVITYELPFAEIVFDFFDRLKSGTKGYASMDYEFIDYR
QSALVKLDILINGEAVDALAVIVHRDKAYHYGRALALKLKRTIPRQMFEVAIQAAIGQKI
IARESISALRKNVTAKCYGGDITRKRKLLEKQKEGKKRMKRMGNVELPQEAFLAALQVGD
E