Protein Info for DvMF_2695 in Desulfovibrio vulgaris Miyazaki F

Annotation: NAD-dependent epimerase/dehydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF04321: RmlD_sub_bind" amino acids 13 to 160 (148 residues), 28.8 bits, see alignment E=9.9e-11 PF01370: Epimerase" amino acids 14 to 245 (232 residues), 231.6 bits, see alignment E=1.4e-72 PF16363: GDP_Man_Dehyd" amino acids 46 to 305 (260 residues), 68.2 bits, see alignment E=1.4e-22

Best Hits

Swiss-Prot: 68% identical to FCL2_ARATH: Putative GDP-L-fucose synthase 2 (GER2) from Arabidopsis thaliana

KEGG orthology group: K02377, GDP-L-fucose synthase [EC: 1.1.1.271] (inferred from 100% identity to dvm:DvMF_2695)

MetaCyc: 68% identical to GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase (Arabidopsis thaliana col)
GDP-L-fucose synthase. [EC: 1.1.1.271]

Predicted SEED Role

"GDP-L-fucose synthetase (EC 1.1.1.271)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 1.1.1.271)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.271

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DIX3 at UniProt or InterPro

Protein Sequence (321 amino acids)

>DvMF_2695 NAD-dependent epimerase/dehydratase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MMQQAPPMQPHSRIYVAGHRGLVGSAIVRALQARGFNDLVLRTHAELDLCDQHAVSAFFA
AEKPEYVFLAAAKVGGIHANDTYPAQFIRDNLLIQTNIIDAAYRNGCKKLLFLGSSCIYP
KLCPQPIKEEYLLTGPLEPTNEWYAIAKIAGIKMCQAYRKQYGFDAISAMPTNLYGPGDN
YHPENSHVIPALIRRFHEAKVAGTDRVKIWGTGAALREFLYVDDLARASVFLMENYSDCE
HVNVGCQEEISIMDVAKCIAGVVGFRGEVVNDPTRPDGTPRKLMDSGRLYAMGWRPSWSL
VDGLRKAYLDFLERHGDVLEG