Protein Info for DvMF_2659 in Desulfovibrio vulgaris Miyazaki F

Annotation: cysteine desulfurase NifS (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 TIGR03402: cysteine desulfurase NifS" amino acids 4 to 382 (379 residues), 647.8 bits, see alignment E=2.3e-199 PF00266: Aminotran_5" amino acids 4 to 364 (361 residues), 310.5 bits, see alignment E=2.5e-96 PF01041: DegT_DnrJ_EryC1" amino acids 44 to 181 (138 residues), 29.8 bits, see alignment E=5.8e-11 PF01212: Beta_elim_lyase" amino acids 46 to 272 (227 residues), 51.9 bits, see alignment E=1e-17

Best Hits

Swiss-Prot: 63% identical to NIFS_TRIAZ: Cysteine desulfurase (nifS) from Trichormus azollae

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 100% identity to dvm:DvMF_2659)

MetaCyc: 49% identical to mitochondrial cysteine desulfurase NFS1 (Homo sapiens)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), NifS subfamily" in subsystem Alanine biosynthesis or Nitrogen fixation (EC 2.8.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DIT7 at UniProt or InterPro

Protein Sequence (404 amino acids)

>DvMF_2659 cysteine desulfurase NifS (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MNTVYFDNNATTRVDPAVLAAMLPYFGELYGNPSSMHTFGGHVGRAVREAREQVARLIGA
TPEEIVFTSCGTEGDNTAIRAALAAQPEKKHIITTRVEHPAVLSLSQHLEKNGYELTLLG
VDDKGRMDLDELREAIRKDTAIVSIMFANNETGTIFPVAEAAAICKERGVPFHTDAVQAV
GKLAIDVRELPVDYLVLSGHKLHAPKGIGALYVRRGAAYRPFLRGGHQERGRRGGTENVP
AIIGLGMACDLAAANMAEENTRVRALRDRLEQGLLAAIPDAIVNGDPANRLPNTTNISFK
YVEGEAILLLLDQLGVCASSGSACTSGSLEPSHVLRAMGVPFTYAHGSIRFSLSRFNTQE
EVDHVVRELPRVIARLREMSPFRAGSEAEAALCKPDRSCQGCGS