Protein Info for DvMF_2620 in Desulfovibrio vulgaris Miyazaki F

Annotation: nitrogenase MoFe cofactor biosynthesis protein NifE (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 TIGR01283: nitrogenase MoFe cofactor biosynthesis protein NifE" amino acids 29 to 463 (435 residues), 608.8 bits, see alignment E=2.8e-187 PF00148: Oxidored_nitro" amino acids 54 to 450 (397 residues), 353.5 bits, see alignment E=7.1e-110

Best Hits

Swiss-Prot: 51% identical to NIFE_METBA: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (Fragment) (nifE2) from Methanosarcina barkeri

KEGG orthology group: K02587, nitrogenase molybdenum-cofactor synthesis protein NifE (inferred from 100% identity to dvm:DvMF_2620)

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifE" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DR81 at UniProt or InterPro

Protein Sequence (474 amino acids)

>DvMF_2620 nitrogenase MoFe cofactor biosynthesis protein NifE (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MTTATAPNATATAECRDADLLDERKKQVHRAGQGPIDMACNRESLAGAVSQRACVFCGSR
VVLYPIADALHLVHGPIGCAAYTWDIRGALSSGPELHRLSFSTDLQEKDVIFGGEKKLAA
ALDELIDLHQPKAAFVYSTCIVGLIGDDLGAVCRKMSEKKGIPVIPVQSEGFKGNKREGY
LAACKAMFRLVGTAPTEDVSPLSVNILGDFNLAGEIWIVRGYLARMGIQVVANITGDGRV
DDIRRCHGAALNLVQCSGSTMDLARMMKEKYGTPHLRVSYLGIEDMADSLYAVAEHFESV
DPGIVERTRELVREELAALMPRLRDIRRDLSGKRAAIYVGGSFKAFSLIKAFRHIGMNVV
LVGSQTGTQEEYRELETVCDPGTVIIDDANPLELSHYVRELDVDVFVGGVKERPIAHKLG
VGFCDHNHERKIALAGFEGMYNFAREVHASVMSPVWRFVPRRARRAAIPAGADA