Protein Info for DvMF_2306 in Desulfovibrio vulgaris Miyazaki F

Annotation: carbamoyl-phosphate synthase, large subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1058 (1057 residues), 1529.8 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 8 to 124 (117 residues), 156.3 bits, E=1.2e-49
  • amino acids 563 to 674 (112 residues), 170.5 bits, E=4.5e-54
• PF02786: CPSase_L_D2
  • amino acids 129 to 334 (206 residues), 233 bits, E=1.1e-72
  • amino acids 678 to 880 (203 residues), 123.2 bits, E=4.5e-39
• PF02787: CPSase_L_D3
  • amino acids 425 to 511 (87 residues), 97.7 bits, E=1.3e-31
• PF02222: ATP-grasp
  • amino acids 685 to 850 (166 residues), 39.1 bits, E=2.6e-13
• PF02655: ATP-grasp_3
  • amino acids 701 to 850 (150 residues), 31.7 bits, E=6.1e-11
• PF07478: Dala_Dala_lig_C
  • amino acids 706 to 848 (143 residues), 24.1 bits, E=9.7e-09
• PF02142: MGS
  • amino acids 961 to 1046 (86 residues), 73.4 bits, E=5.4e-24

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (16/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• superpathway of arginine and polyamine biosynthesis (10/17 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (4/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See B8DQX8 at UniProt or InterPro

Protein Sequence (1078 amino acids)

>DvMF_2306 carbamoyl-phosphate synthase, large subunit (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MPKRTDLKRIMVIGSGPIVIGQACEFDYSGTQAVKALKEEGYEVVLVNSNPATIMTDPGL
ADRTYIEPIEPETVAAIIRKERPDALLPTLGGQTGLNTALALAAMGVLEECGVDLIGANK
QVIEKAESRELFREAMANIGLKVPASGIARTMEDVRRLGTEMPFPLIIRPAFTMGGTGGG
IAYNMEDLEEIAARGLAASIKHEVMIEQSVLGWKEFEMEVMRDKADNCVIICSIENFDPM
GVHTGDSITVAPAQTLTDVEYQMMRDASIAIMREIGVETGGSNVQFGINPQNGDMVVIEM
NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDEIPNDITRETMASFEPSIDYCVTKIPR
FTFEKFPGAKDELTTSMKSVGEAMSIGRTFKESLQKGLRSLEVGAPGLGSAFRCKGPERE
DIMRKLRTPNSRRIFYVRHAMLDGMTVEEIHEATAIDPWFLRQMKDLVDMEAELRDFALG
NAMTVDNAELVALMRRAKEYGFSDRQLAEMWKRPESDVRTLRKQMGIAPTYYLVDTCASE
FEAYTPYYYSTYETGHEVAVEDRKKVIILGGGPNRIGQGIEFDYCCCHASFALKEMGVQA
IMVNSNPETVSTDYDTSDRLYFEPLTFEDVMNIIETEKPDGVVVQFGGQTPLNLAVPLMR
AGVPILGTSPDSIDRAEDRERFQALLQKLGLRQPANATVMSLPEARSAAARIGYPTVVRP
SYVLGGRAMEIVYDEEQLAEYFANSVGEKPKHPILIDKFLESAIEVDVDALSDGTDVYVA
GIMEHIEEAGIHSGDSACVIPPHTLPEAIVNEIARQTVELARELRVVGLMNIQFAVKDGL
IYILEVNPRASRTAPFVSKATAVPLPRLATQIMLGATLKELDPWSMRRTGYFSVKESVFP
FNRFPGVDILLGPEMRSTGEVMGIASSFEEAYLKGQLAGGQRLPQSGKIFISVNDRDKLL
ITEVASMFADLGFEVLATSGTAKLLREGGVPATSVHKVYEGRPNIVDFIKNGDIALVLNT
ASGKRTVQDSKSIRQATLMYGVPYSTTVSGAKAIAQAIRASRCCGVNVKSLQEYYGTN