Protein Info for DvMF_2084 in Desulfovibrio vulgaris Miyazaki F

Annotation: DNA gyrase, A subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 TIGR01063: DNA gyrase, A subunit" amino acids 4 to 802 (799 residues), 1139.7 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 26 to 465 (440 residues), 560.3 bits, see alignment E=3e-172 PF03989: DNA_gyraseA_C" amino acids 498 to 543 (46 residues), 40.5 bits, see alignment 1.5e-14 amino acids 548 to 597 (50 residues), 30.6 bits, see alignment 1.8e-11 amino acids 605 to 643 (39 residues), 25.5 bits, see alignment 7.2e-10 amino acids 653 to 697 (45 residues), 41.3 bits, see alignment 8.3e-15 amino acids 706 to 752 (47 residues), 38.6 bits, see alignment 5.8e-14 amino acids 756 to 802 (47 residues), 34.3 bits, see alignment 1.3e-12

Best Hits

Swiss-Prot: 75% identical to GYRA_LAWIP: DNA gyrase subunit A (gyrA) from Lawsonia intracellularis (strain PHE/MN1-00)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 100% identity to dvm:DvMF_2084)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DQA8 at UniProt or InterPro

Protein Sequence (812 amino acids)

>DvMF_2084 DNA gyrase, A subunit (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MSQQVSIEKELRKSYLEYSLSVIIGRAIPDARDGLKPVHRRIMFAQHELGNGYTRPPKKS
ARVVGDVIGKYHPHGDSAVYDALVRMAQDFSMRDPLVDGQGNFGSIDGDAAAAMRYTEVR
MSRLAGEFLADIDKETVDFRPNYDNTLLEPSVLPTKVPNLLLNGSSGIAVGMATNIPPHN
LGELCGALLHIVDEPGCTVEDLMDHVKGPDFPTAGFVYAGQGLYDAYTTGRGTVKVRGKV
EIEERKKGLQAIVIREIPFALNKSSLVEKIAALVNERKIDGVADLRDESDRKGIRVVIDL
KRGTIPDIVVNALYKYTPLESSFGINMLAVVGNRPQLLNLKQALEIFLEHRREVIIRRTR
YDLRKAEARAHILEGLRIALDNIDEVVALIRASKTPQDAKDALMGRFTLSEAQAQAILDM
RLQRLTNLEHEKLIEEYNELLKKIEFFRSVLENPEVLRSVIREEIGELRDTFATKRRTEV
LEEELDGIDIEDLIPDEDVVITLSRRGYIKRTTLDNYQQQRRGGKGIAGVHTSEDDFVQD
FLTTTNHQYLLLFTNHGRMHQIKVHRVPEGSRTAKGVHIANLVQLDKDELVTNVLTVREF
AEDRYFLFVTRRGMVKRSSASLYAKCRKTGLIAVGLRPDDELIMVREVDEHCHIVLTTGD
GMAIRFQCEDVRPMGRSATGVKGIALRGKDRVVACVILAEEDTTSMIMSVSANGFGKRTK
ADLYRVQSRGGVGVKNFKVTPKTGDVIGAMPVRDDDALVLLTSTNKILRMGLNDVRSVGR
ATQGVRLVRLDEGALVVGFDRIDEREVVDGEE