Protein Info for DvMF_1791 in Desulfovibrio vulgaris Miyazaki F

Annotation: 2-isopropylmalate synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 TIGR00973: 2-isopropylmalate synthase" amino acids 7 to 500 (494 residues), 721.1 bits, see alignment E=2.8e-221 PF00682: HMGL-like" amino acids 8 to 264 (257 residues), 318.1 bits, see alignment E=5e-99 PF08502: LeuA_dimer" amino acids 372 to 502 (131 residues), 120.6 bits, see alignment E=4.3e-39

Best Hits

Swiss-Prot: 100% identical to LEU1_DESVM: 2-isopropylmalate synthase (leuA) from Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to dvm:DvMF_1791)

MetaCyc: 50% identical to 2-isopropylmalate synthase (Escherichia coli K-12 substr. MG1655)
2-isopropylmalate synthase. [EC: 2.3.3.13]

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DM91 at UniProt or InterPro

Protein Sequence (514 amino acids)

>DvMF_1791 2-isopropylmalate synthase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MTIESGRIRIFDTTLRDGEQSPGATMNLQEKIRLARQLETLGVDIMEAGFPASSQGDFEA
VQAIARAVKGVEVAGLCRAMPADIDRAWEAVKVAENPRIHTFLATSPVHMQYKLRKEPDQ
VVEMAVAAVRHAAKYTSNVEFSAEDASRSNPDFLVRVFEAVINAGATTINVPDTVGYAQP
EEFGRLIRYVIENTPNSHKAVFSVHCHNDLGMGVANTLAALKAGARQAEVTISGIGERAG
NASLEEIVMALHTRRDFYQLDCGVVTEQLFPTCRLLSMIIGQPIPPNKAIVGANAFAHES
GIHQDGMLKNRETYEIMTPESIGKTKTDLVIGKHSGRNAVKNKLDELGYRLEEAQLVTVF
EAVKKLADKKKQIYDEDIEALVLEEVYRLPDLYRLVNLSVQCSDTGMPPTAAVVMDVMGE
VKRAAGFGVGPIDAVFNVIGEIVGRAPVLERYSVTAITGGTDAQGEVTVRLRQNGSSAVG
RGSDPDIILASARAYVNALNRLAKKEEEQEKEGI