Protein Info for DvMF_1745 in Desulfovibrio vulgaris Miyazaki F

Annotation: glutamine amidotransferase of anthranilate synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00566: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
  • amino acids 1 to 185 (185 residues), 192.9 bits, E=2.5e-61
• PF00117: GATase
  • amino acids 3 to 185 (183 residues), 163.9 bits, E=3.5e-52
• PF07722: Peptidase_C26
  • amino acids 72 to 171 (100 residues), 29.4 bits, E=6.9e-11

Best Hits

Swiss-Prot: 49% identical to PABA_BACSU: Aminodeoxychorismate/anthranilate synthase component 2 (pabA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01658, anthranilate synthase component II [EC: 4.1.3.27] (inferred from 100% identity to dvm:DvMF_1745)

MetaCyc: 49% identical to 4-amino-4-deoxychorismate synthase; anthranilate synthase (subunit II) (Bacillus subtilis subtilis 168)
RXN-22440 [EC: 2.6.1.123]

Predicted SEED Role

"Anthranilate synthase, amidotransferase component (EC 4.1.3.27)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. or Tryptophan synthesis (EC 4.1.3.27)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (17/18 steps found)
• superpathway of L-tryptophan biosynthesis (12/13 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• 4-aminobenzoate biosynthesis II (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (8/12 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• superpathway of tetrahydrofolate biosynthesis (6/10 steps found)
• 4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• acridone alkaloid biosynthesis (1/4 steps found)
• superpathway of candicidin biosynthesis (3/11 steps found)
• superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
• chloramphenicol biosynthesis (1/9 steps found)
• superpathway of chorismate metabolism (31/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.27

Use Curated BLAST to search for 2.6.1.123 or 4.1.3.27

Sequence Analysis Tools

See B8DM46 at UniProt or InterPro

Protein Sequence (192 amino acids)

>DvMF_1745 glutamine amidotransferase of anthranilate synthase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MFLLIDNYDSFTFNLVQAFYGLGLHPVVVRNDDPAVPAMAEDPALEMVCISPGPSHPRNA
GFCLDFLSRLPHRVPVLGVCLGHQVLGLFAGATVDVGPRIMHGKTSDITHDGQGLFHGVP
SPMQVGRYHSLIVHAEERPDLLAVTARAPEGEVMALRYTDRPWVGVQFHPESVLTPDGVR
MLANFPAHVAGR