Protein Info for DvMF_1312 in Desulfovibrio vulgaris Miyazaki F

Annotation: NAD-dependent epimerase/dehydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 13 to 281 (269 residues), 32.8 bits, E=1.2e-11
• PF01370: Epimerase
  • amino acids 14 to 242 (229 residues), 174 bits, E=1.1e-54
• PF01073: 3Beta_HSD
  • amino acids 15 to 228 (214 residues), 27.7 bits, E=4.3e-10
• PF16363: GDP_Man_Dehyd
  • amino acids 15 to 312 (298 residues), 190.2 bits, E=2.1e-59
• PF07993: NAD_binding_4
  • amino acids 75 to 199 (125 residues), 22.9 bits, E=1.3e-08

Best Hits

Swiss-Prot: 62% identical to UXS5_ARATH: UDP-glucuronic acid decarboxylase 5 (UXS5) from Arabidopsis thaliana

KEGG orthology group: K01710, dTDP-glucose 4,6-dehydratase [EC: 4.2.1.46] (inferred from 100% identity to dvm:DvMF_1312)

MetaCyc: 64% identical to UDP-xylose synthase 1 subunit (Sinorhizobium meliloti 1021)
UDP-glucuronate decarboxylase. [EC: 4.1.1.35]

Predicted SEED Role

"NAD-dependent epimerase/dehydratase family protein"

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• UDP-α-D-xylose biosynthesis (2/2 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-β-D-fucofuranose biosynthesis (2/4 steps found)
• dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
• dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
• dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-α-D-fucose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
• dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
• dTDP-β-L-olivose biosynthesis (3/7 steps found)
• dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (2/6 steps found)
• dTDP-D-desosamine biosynthesis (2/6 steps found)
• dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
• dTDP-β-L-megosamine biosynthesis (3/8 steps found)
• dTDP-β-L-mycarose biosynthesis (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• dTDP-α-D-forosamine biosynthesis (3/9 steps found)
• dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (3/9 steps found)
• superpathway of enterobacterial common antigen biosynthesis (2/10 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of erythromycin biosynthesis (2/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• superpathway of megalomicin A biosynthesis (3/22 steps found)
• superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (3/23 steps found)

KEGG Metabolic Maps

• Biosynthesis of vancomycin group antibiotics
• Nucleotide sugars metabolism
• Polyketide sugar unit biosynthesis
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.46

Use Curated BLAST to search for 4.1.1.35 or 4.2.1.46

Sequence Analysis Tools

See B8DLJ9 at UniProt or InterPro

Protein Sequence (330 amino acids)

>DvMF_1312 NAD-dependent epimerase/dehydratase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MQQGEFKELARKRILVTGGAGFIGSHLCRRLLDRGAEVLCVDNFFTGSRDHVQEMQDHPR
FELLRHDITFPLYVEVDEIYNLACPASPIHYQFDPVQTTKTCVHGSINMLGLAKRVKARI
LQASTSEVYGDPETHPQTEDYWGRVNPIGPRSCYDEGKRCAETLFTDYHRQHGVPIRIAR
IFNTFGPRMHPNDGRVVSNFILQALQDKPITIYGDGSQTRSFCYVDDMVDGLTALMHAPD
DAHLPVNLGNPEERTILNLAEIIIEFVNSRSTIDFRPLPQDDPRRRRPDIAQAREKLGWE
PKVSMEEGLRKTVEYFEGLLRSRRAEGAEV