Protein Info for DvMF_0960 in Desulfovibrio vulgaris Miyazaki F

Annotation: UDP-N-acetylmuramyl-tripeptide synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 19 to 491 (473 residues), 465.6 bits, see alignment E=1e-143 PF08245: Mur_ligase_M" amino acids 114 to 319 (206 residues), 167.6 bits, see alignment E=3.6e-53 PF02875: Mur_ligase_C" amino acids 339 to 424 (86 residues), 83.4 bits, see alignment E=1.1e-27

Best Hits

Swiss-Prot: 60% identical to MURE_LAWIP: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Lawsonia intracellularis (strain PHE/MN1-00)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to dvm:DvMF_0960)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DP83 at UniProt or InterPro

Protein Sequence (494 amino acids)

>DvMF_0960 UDP-N-acetylmuramyl-tripeptide synthetase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MTRISFDGLLARVRENTPEIRTDSRKVGRGDVFVAVPGVAANGGAGVDGADFIVKAWAAG
AGAVVCLPARVEAALADAPEGAVVAAHDDPRVAIGLLGQARYGTDALPFPVVAVTGTNGK
TTITYLLDHVFSALGRRAGVLGTVSYRWPGFAMDAPLTTPDCIETHAMLGRMRDAGVDVA
LMEVSSHALDQRRVAGIRFAGAILTNLTQDHLDYHGDMEHYYAAKARLFTELPDADKACA
VNADDAWGRRLLGEVPHAIGFGLDAGNAVSGRRFLHGEMVRNATSGLTLRMTYEGRQWEL
SSHLVGAHNASNLLAVQAVCLGMGLAPQDFASLAGFTGVPGRLERIVNPRGLDIFVDYAH
TPDALENVLRALRAVGFSRIVTVFGCGGNRDRTKRPLMGQAVARHADVAVLTSDNPRHED
PEAIMADVLPGLAGARKVVSDADRARAIGKALALLQPGDVLLVAGKGHESYQQIGDRKVP
YSDQQVIREILGCN