Protein Info for DvMF_0565 in Desulfovibrio vulgaris Miyazaki F
Name: aspA
Annotation: aspartate ammonia-lyase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to ASPA_PSEAE: Aspartate ammonia-lyase (aspA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 100% identity to dvm:DvMF_0565)MetaCyc: 44% identical to fumarase subunit (Ascaris suum)
Fumarate hydratase. [EC: 4.2.1.2]
Predicted SEED Role
"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)
MetaCyc Pathways
- reductive TCA cycle I (9/11 steps found)
- incomplete reductive TCA cycle (6/7 steps found)
- L-glutamate degradation II (2/2 steps found)
- TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
- TCA cycle VI (Helicobacter) (7/9 steps found)
- L-aspartate degradation II (aerobic) (2/3 steps found)
- L-aspartate degradation III (anaerobic) (2/3 steps found)
- TCA cycle VII (acetate-producers) (6/9 steps found)
- mixed acid fermentation (11/16 steps found)
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (18/26 steps found)
- partial TCA cycle (obligate autotrophs) (5/8 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (15/22 steps found)
- superpathway of L-aspartate and L-asparagine biosynthesis (2/4 steps found)
- TCA cycle I (prokaryotic) (6/10 steps found)
- TCA cycle III (animals) (6/10 steps found)
- TCA cycle VIII (Chlamydia) (3/6 steps found)
- TCA cycle II (plants and fungi) (5/9 steps found)
- TCA cycle IV (2-oxoglutarate decarboxylase) (5/9 steps found)
- reductive TCA cycle II (7/12 steps found)
- superpathway of glyoxylate bypass and TCA (7/12 steps found)
- pyruvate fermentation to propanoate I (2/7 steps found)
- L-canavanine degradation II (1/6 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (6/13 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (3/10 steps found)
- superpathway of glyoxylate cycle and fatty acid degradation (5/14 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (7/17 steps found)
- methylaspartate cycle (8/19 steps found)
- L-glutamate degradation VIII (to propanoate) (2/11 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Nitrogen metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.2, 4.3.1.1
Use Curated BLAST to search for 4.2.1.2 or 4.3.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B8DKU3 at UniProt or InterPro
Protein Sequence (521 amino acids)
>DvMF_0565 aspartate ammonia-lyase (RefSeq) (Desulfovibrio vulgaris Miyazaki F) MTSLMEYRIERDSLGERKVPAHAYYGVQTLRALENFDVTGLTIGRHPRFVQALASVKKAA ALANMELGLLDGDVGQAIVQACREVREGRFDEQFVVDIIQGGAGTSVNMNANEVIANRAL ELMGRTRGEYGVVSPLNHVNLSQSTNDVYPTAFRIALVWYARDLGVSLRELRDAFHAKGE AFADVIKMARTQLQDAVPITLGAEFAAYGVTVGEDIDRLAEVARLLCEVNIGATAVGTGI NTVPGYAPLVCDRLADITGLPLSLSPNLVEATSDAGAYVTLSGLLKRVAVKLSKICNDLR LLSSGPFTGLGEISLPAVQPGSSIMPGKVNPVIPELVNQVCQQVIGNDLTVTLAAEAGQL ELNVFGPIMACNLFQSLEILTRAARILRVRCVEGITANRERCLELLHGSLGMVTALAPVI GYEAAATVVKDARATGRTPRDLLVERGLMRSEDYDDLMDPAKMLGPRDVRGHRNGALRQD AAGREAVGKGTAEQGAGQDGAYGPAGRDGPDGPGEPGEEDA