Protein Info for Shewana3_4045 in Shewanella sp. ANA-3

Annotation: hydrophobe/amphiphile efflux-1 (HAE1) family protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1044 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 339 to 359 (21 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details amino acids 394 to 414 (21 residues), see Phobius details amino acids 438 to 458 (21 residues), see Phobius details amino acids 470 to 494 (25 residues), see Phobius details amino acids 538 to 556 (19 residues), see Phobius details amino acids 870 to 888 (19 residues), see Phobius details amino acids 895 to 915 (21 residues), see Phobius details amino acids 922 to 942 (21 residues), see Phobius details amino acids 970 to 990 (21 residues), see Phobius details amino acids 1002 to 1024 (23 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1024 (1024 residues), 1331.9 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1038 (1038 residues), 1735.2 bits, see alignment E=0 PF03176: MMPL" amino acids 297 to 496 (200 residues), 33.3 bits, see alignment E=4e-12

Best Hits

Swiss-Prot: 66% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 66% identity to abo:ABO_0964)

MetaCyc: 66% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L2J3 at UniProt or InterPro

Protein Sequence (1044 amino acids)

>Shewana3_4045 hydrophobe/amphiphile efflux-1 (HAE1) family protein (RefSeq) (Shewanella sp. ANA-3)
MARFFIDRPIFAWVIALIIMLAGVLSIRSLPVSQYPSIAPPTVVISANYPGASAKIVEDS
VTQVIEQRMKGIDHLRYIASTSDSFGNASITLTFNAEADPDIAQVQVQNKLQGAMTLLPQ
EVQSQGVNVNKSSSGFLMVLGFVSTDGSLDKGDIADYVGANIQDPMSRVPGVGEIQIFGA
QYAMRIWLDPLKLTQYNLTSLDVVSAIRAQNAQVSAGQLGGTPSLPGQELNATVSAQSRL
QTPEQFRKIIIKSDTSGANVFLGDVARVELGSESYAVESLYNGKPASGIAIKLATGANAL
DTAEAVRAKVEELRPFFPAGLDVVYPYDTTPFVEKSIEGVVHTLLEAIVLVFVIMYLFLQ
NFRATLIPTIAVPVVLLGTFAILSATGFSINTLTMFAMVLAIGLLVDDAIVVVENVERVM
AEEGLSPLEATRKSMDQITGALVGIGLTLSAVFVPMAFMSGSTGVIYRQFSITIVSAMAL
SVLVALILTPALCATMLKPLKKGHGHIETGFFGWFNRGFDRLTNRYESSVAGIIKRSFRV
MMIYAALVVAVGWIFMRMPTAFLPDEDQGILFTQAILPTNSTQESTLKVLEKVSDHYLAE
EGVRSVFSVAGFSFAGQGQNMGIAFVGLKDWSEREAPGMDVQSIAGRAMGVFSQMKEAFV
FAFVPPAVIELGTANGFDMYLQDKNGQGHDKLVAARNQLLGMAAQNPNLVGVRPNGQEDA
PIYQLHIDHAKLSALGIEIANVNSVLATAWGGSYVNDFIDRGRVKKVYVQGDAQYRMQPE
DLDTWYVRNNKGDMVPFSAFATGAWEYGSPRLERFNGLPAMNIQGATAPGFSTGAAMNIM
EDMVKQLPPGFGIEWNGLSYEERLSGNQAPALYALSILVVFLVLAALYESWSVPFAVILV
VPLGIIGALLAMNGRGLPNDVFFQVGLLTTVGLATKNAILIVEFAKEFYEKGAGLIEATL
HAVRVRLRPILMTSLAFGLGVVPLAISTGVGSGSQNAIGTGVLGGMMSSTFLGIFFVPLF
FVIVERIFSKREKKGKEEKPTSAE